Publication:
Influence of a fat overload on lipogenic regulators in metabolic syndrome patients

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Abstract
Several epidemiological studies have related an increase of lipids in the postprandial state to an individual risk for the development of CVD, possibly due to the increased plasma levels of TAG and fatty acids (FA) through enzymes of FA metabolism. The interaction between nutrition and the human genome determines gene expression and metabolic response. The aim of the present study was to evaluate the influence of a fat overload on the gene mRNA levels of lipogenic regulators in peripheral blood mononuclear cells (PBMC) from patients with the metabolic syndrome. The study included twenty-one patients with criteria for the metabolic syndrome who underwent a fat overload. Measurements were made before and after the fat overload of anthropometric and biochemical variables and also the gene mRNA levels of lipogenic factors. The main results were that the fat overload led to an increased mRNA levels of sterol regulatory element binding protein-1 (SREBP1), retinoid X receptor α (RXRα) and liver X receptor α (LXRα) in PBMC, and this increase was associated with the FA synthase (FASN) mRNA levels. We also found that TAG levels correlated with FASN mRNA levels. In addition, there was a positive correlation of SREBP1 with RXRα and of LXRα with the plasma lipoperoxide concentration. The fat overload led to an increase in regulators of lipogenesis in PBMC from patients with the metabolic syndrome.
Description
Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't;
MeSH Terms
Medical Subject Headings::Chemicals and Drugs::Macromolecular Substances::Multiprotein Complexes::Multienzyme Complexes::Fatty Acid Synthetase Complex
Medical Subject Headings::Check Tags::Female
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Anatomy::Cells::Blood Cells::Leukocytes::Leukocytes, Mononuclear
Medical Subject Headings::Chemicals and Drugs::Organic Chemicals::Free Radicals::Peroxides::Lipid Peroxides
Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Biochemical Processes::Lipogenesis
Medical Subject Headings::Check Tags::Male
Medical Subject Headings::Diseases::Nutritional and Metabolic Diseases::Metabolic Diseases::Metabolic Syndrome X
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::DNA-Binding Proteins::Receptors, Cytoplasmic and Nuclear::Orphan Nuclear Receptors
Medical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::RNA::RNA, Messenger
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::DNA-Binding Proteins::Receptors, Cytoplasmic and Nuclear::Receptors, Retinoic Acid::Retinoid X Receptors
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::DNA-Binding Proteins::Basic Helix-Loop-Helix Transcription Factors::Basic Helix-Loop-Helix Leucine Zipper Transcription Factors::Sterol Regulatory Element Binding Proteins
Medical Subject Headings::Chemicals and Drugs::Lipids::Glycerides::Triglycerides
Medical Subject Headings::Technology, Industry, Agriculture::Food and Beverages::Food::Dietary Fats
DeCS Terms
CIE Terms
Keywords
Complejo Ácido Graso Sintetasa, Femenino, Humanos, Leucocitos Mononucleares, Peróxidos Lipídicos, Lipogénesis, Masculino, Síndrome X Metabólico, Receptores Nucleares Huérfanos, ARN Mensajero, Receptores X Retinoide, Proteínas de Unión a los Elementos Reguladores de Esteroles, Triglicéridos, Grasas en la Dieta, Genetic expression, Postprandial hypertriacylglycerolaemia, Lipogenic genes
Citation
Mayas MD, Queipo-Ortuño MI, Clemente-Postigo M, Macias M, El Bekay R, Tinahones FJ, Cardona F. Influence of a fat overload on lipogenic regulators in metabolic syndrome patients. Br. J. Nutr.. 2011; 105(6):895-901