Please use this identifier to cite or link to this item:
Title: Glutathione reductase gsr-1 is an essential gene required for Caenorhabditis elegans early embryonic development.
Authors: Mora-Lorca, José Antonio
Sáenz-Narciso, Beatriz
Gaffney, Christopher J
Naranjo-Galindo, Francisco José
Pedrajas, José Rafael
Guerrero-Gómez, David
Dobrzynska, Agnieszka
Askjaer, Peter
Szewczyk, Nathaniel J
Cabello, Juan
Miranda-Vizuete, Antonio
Keywords: Caenorhabditis elegans;Embryonic development;Glutathione reductase;Mitochondria;Redox
metadata.dc.subject.mesh: Animals
Caenorhabditis elegans
Embryonic Development
Genes, Essential
Glutathione Reductase
Mutant Proteins
Protein Isoforms
Issue Date: 24-Apr-2016
Abstract: Glutathione is the most abundant thiol in the vast majority of organisms and is maintained in its reduced form by the flavoenzyme glutathione reductase. In this work, we describe the genetic and functional analysis of the Caenorhabditis elegans gsr-1 gene that encodes the only glutathione reductase protein in this model organism. By using green fluorescent protein reporters we demonstrate that gsr-1 produces two GSR-1 isoforms, one located in the cytoplasm and one in the mitochondria. gsr-1 loss of function mutants display a fully penetrant embryonic lethal phenotype characterized by a progressive and robust cell division delay accompanied by an aberrant distribution of interphasic chromatin in the periphery of the cell nucleus. Maternally expressed GSR-1 is sufficient to support embryonic development but these animals are short-lived, sensitized to chemical stress, have increased mitochondrial fragmentation and lower mitochondrial DNA content. Furthermore, the embryonic lethality of gsr-1 worms is prevented by restoring GSR-1 activity in the cytoplasm but not in mitochondria. Given the fact that the thioredoxin redox systems are dispensable in C. elegans, our data support a prominent role of the glutathione reductase/glutathione pathway in maintaining redox homeostasis in the nematode.
metadata.dc.identifier.doi: 10.1016/j.freeradbiomed.2016.04.017
Appears in Collections:Producción 2020

Files in This Item:
There are no files associated with this item.

This item is protected by original copyright

This item is licensed under a Creative Commons License Creative Commons