Please use this identifier to cite or link to this item:
Title: RPL13A and EEF1A1 Are Suitable Reference Genes for qPCR during Adipocyte Differentiation of Vascular Stromal Cells from Patients with Different BMI and HOMA-IR.
Authors: Gentile, Adriana-Mariel
Lhamyani, Said
Coín-Aragüez, Leticia
Oliva-Olivera, Wilfredo
Zayed, Hatem
Vega-Rioja, Antonio
Monteseirin, Javier
Romero-Zerbo, Silvana-Yanina
Tinahones, Francisco-José
Bermúdez-Silva, Francisco-Javier
El Bekay, Rajaa
metadata.dc.subject.mesh: Adipocytes
Body Mass Index
Cell Differentiation
Cells, Cultured
Gene Expression Profiling
Insulin Resistance
Middle Aged
Peptide Elongation Factor 1
Reference Standards
Reverse Transcriptase Polymerase Chain Reaction
Ribosomal Proteins
Stromal Cells
Young Adult
Issue Date: 15-Jun-2016
Abstract: Real-time or quantitative PCR (qPCR) is a useful technique that requires reliable reference genes for data normalization in gene expression analysis. Adipogenesis is among the biological processes suitable for this technique. The selection of adequate reference genes is essential for qPCR gene expression analysis of human Vascular Stromal Cells (hVSCs) during their differentiation into adipocytes. To the best of our knowledge, there are no studies validating reference genes for the analyses of visceral and subcutaneous adipose tissue hVSCs from subjects with different Body Mass Index (BMI) and Homeostatic Model Assessment of Insulin Resistance (HOMA-IR) index. The present study was undertaken to analyze this question. We first analyzed the stability of expression of five potential reference genes: CYC, GAPDH, RPL13A, EEF1A1, and 18S ribosomal RNA, during in vitro adipogenic differentiation, in samples from these types of patients. The expression of RPL13A and EEF1A1 was not affected by differentiation, thus being these genes the most stable candidates, while CYC, GAPDH, and 18S were not suitable for this sort of analysis. This work highlights that RPL13A and EEF1A1 are good candidates as reference genes for qPCR analysis of hVSCs differentiation into adipocytes from subjects with different BMI and HOMA-IR.
metadata.dc.identifier.doi: 10.1371/journal.pone.0157002
Appears in Collections:Producción 2020

Files in This Item:
File SizeFormat 
PMC4909211.pdf3,13 MBAdobe PDFView/Open

This item is protected by original copyright

This item is licensed under a Creative Commons License Creative Commons