Please use this identifier to cite or link to this item:
Title: Identification and characterization of a bacterial hyaluronidase and its production in recombinant form.
Authors: Messina, Luciano
Gavira, Jose A
Pernagallo, Salvatore
Unciti-Broceta, Juan D
Sanchez Martin, Rosario M
Diaz-Mochon, Juan J
Vaccaro, Susanna
Conejero-Muriel, Mayte
Pineda-Molina, Estela
Caruso, Salvatore
Musumeci, Luca
Di Pasquale, Roberta
Pontillo, Angela
Sincinelli, Francesca
Pavan, Mauro
Secchieri, Cynthia
Keywords: Streptomyces koganeiensis;hyaluronic acid or hyaluronan;hyaluronidase
metadata.dc.subject.mesh: Bacterial Proteins
Enzyme Stability
Recombinant Proteins
Issue Date: 4-Jul-2016
Abstract: Hyaluronidases (Hyals) are broadly used in medical applications to facilitate the dispersion and/or absorption of fluids or medications. This study reports the isolation, cloning, and industrial-scale recombinant production, purification and full characterization, including X-ray structure determination at 1.45 Å, of an extracellular Hyal from the nonpathogenic bacterium Streptomyces koganeiensis. The recombinant S. koganeiensis Hyal (rHyal_Sk) has a novel bacterial catalytic domain with high enzymatic activity, compared with commercially available Hyals, and is more thermostable and presents higher proteolytic resistance, with activity over a broad pH range. Moreover, rHyal_Sk exhibits remarkable substrate specificity for hyaluronic acid (HA) and poses no risk of animal cross-infection.
metadata.dc.identifier.doi: 10.1002/1873-3468.12258
Appears in Collections:Producción 2020

Files in This Item:
There are no files associated with this item.

This item is protected by original copyright

Except where otherwise noted, Items on the Andalusian Health Repository site are licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives License.