Please use this identifier to cite or link to this item:
Title: VAMP2 is implicated in the secretion of antibodies by human plasma cells and can be replaced by other synaptobrevins.
Authors: Gómez-Jaramillo, Laura
Romero-García, Raquel
Jiménez-Gómez, Gema
Riegle, Lisa
Ramos-Amaya, Ana Belén
Brieva, José Antonio
Kelly-Worden, Marie
Campos-Caro, Antonio
Keywords: VAMP proteins;exocytosis;human;immunoglobulin;plasma cells
metadata.dc.subject.mesh: Antibodies
Cell Line
Cytoplasmic Vesicles
Gene Silencing
Immunoglobulin E
Plasma Cells
Protein Domains
Protein Transport
Qa-SNARE Proteins
Qb-SNARE Proteins
Qc-SNARE Proteins
RNA, Small Interfering
Tetanus Toxin
Vesicle-Associated Membrane Protein 2
Issue Date: 12-Sep-2016
Abstract: The production and secretion of antibodies by human plasma cells (PCs) are two essential processes of humoral immunity. The secretion process relies on a group of proteins known as soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs), which are located in the plasma membrane (t-SNAREs) and in the antibody-carrying vesicle membrane (v-SNARE), and mediate the fusion of both membranes. We have previously shown that SNAP23 and STX4 are the t-SNAREs responsible for antibody secretion. Here, using human PCs and antibody-secreting cell lines, we studied and characterized the expression and subcellular distribution of vesicle associated membrane protein (VAMP) isoforms, demonstrating that all isoforms (with the exception of VAMP1) are expressed by the referenced cells. Furthermore, the functional role in antibody secretion of each expressed VAMP isoform was tested using siRNA. Our results show that VAMP2 may be the v-SNARE involved in vesicular antibody release. To further support this conclusion, we used tetanus toxin light chain to cleave VAMP2, conducted experiments to verify co-localization of VAMP2 in antibody-carrying vesicles, and demonstrated the coimmunoprecipitation of VAMP2 with STX4 and SNAP23 and the in situ interaction of VAMP2 with STX4. Taken together, these findings implicate VAMP2 as the main VAMP isoform functionally involved in antibody secretion.
metadata.dc.identifier.doi: 10.1038/cmi.2016.46
Appears in Collections:Producción 2020

Files in This Item:
There are no files associated with this item.

This item is protected by original copyright

Except where otherwise noted, Items on the Andalusian Health Repository site are licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives License.