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Title: Soluble phospho-tau from Alzheimer's disease hippocampus drives microglial degeneration.
Authors: Sanchez-Mejias, Elisabeth
Navarro, Victoria
Jimenez, Sebastian
Sanchez-Mico, Maria
Sanchez-Varo, Raquel
Nuñez-Diaz, Cristina
Trujillo-Estrada, Laura
Davila, Jose Carlos
Vizuete, Marisa
Gutierrez, Antonia
Vitorica, Javier
Keywords: Alzheimer;Hippocampus;Human brain;Microglia;Pathology
metadata.dc.subject.mesh: Adult
Aged, 80 and over
Alzheimer Disease
Amyloid beta-Protein Precursor
Antigens, CD
Antigens, Differentiation, Myelomonocytic
Calcium-Binding Proteins
Cells, Cultured
DNA-Binding Proteins
Disease Progression
Gene Expression Regulation
Leukocyte Common Antigens
Mice, Transgenic
Microfilament Proteins
Middle Aged
Receptors, Purinergic P2Y12
tau Proteins
Issue Date: 14-Oct-2016
Abstract: The role of microglial cells in the development and progression of Alzheimer's disease (AD) has not been elucidated. Here, we demonstrated the existence of a weak microglial response in human AD hippocampus which is in contrast to the massive microglial activation observed in APP-based models. Most importantly, microglial cells displayed a prominent degenerative profile (dentate gyrus > CA3 > CA1 > parahippocampal gyrus), including fragmented and dystrophic processes with spheroids, a reduced numerical density, and a significant decrease in the area of surveillance ("microglial domain"). Consequently, there was a substantial decline in the area covered by microglia which may compromise immune protection and, therefore, neuronal survival. In vitro experiments demonstrated that soluble fractions (extracellular/cytosolic) from AD hippocampi were toxic for microglial cells. This toxicity was abolished by AT8 and/or AT100 immunodepletion, validating that soluble phospho-tau was the toxic agent. These results were reproduced using soluble fractions from phospho-tau-positive Thy-tau22 hippocampi. Cultured microglial cells were not viable following phagocytosis of SH-SY5Y cells expressing soluble intracellular phospho-tau. Because the phagocytic capacity of microglial cells is highly induced by apoptotic signals in the affected neurons, we postulate that accumulation of intraneuronal soluble phospho-tau might trigger microglial degeneration in the AD hippocampus. This microglial vulnerability in AD pathology provides new insights into the immunological mechanisms underlying the disease progression and highlights the need to improve or develop new animal models, as the current models do not mimic the microglial pathology observed in the hippocampus of AD patients.
metadata.dc.identifier.doi: 10.1007/s00401-016-1630-5
Appears in Collections:Producción 2020

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