Please use this identifier to cite or link to this item:
Title: Bioactive Polymeric Nanoparticles for Periodontal Therapy.
Authors: Osorio, Raquel
Alfonso-Rodríguez, Camilo Andrés
Medina-Castillo, Antonio L
Alaminos, Miguel
Toledano, Manuel
metadata.dc.subject.mesh: Biomimetic Materials
Calcium Phosphates
Materials Testing
Microscopy, Electron, Scanning
Mouth Mucosa
Periodontal Diseases
Surface Properties
X-Ray Diffraction
Issue Date: 7-Nov-2016
Abstract: to design calcium and zinc-loaded bioactive and cytocompatible nanoparticles for the treatment of periodontal disease. PolymP-nActive nanoparticles were zinc or calcium loaded. Biomimetic calcium phosphate precipitation on polymeric particles was assessed after 7 days immersion in simulated body fluid, by scanning electron microscopy attached to an energy dispersive analysis system. Amorphous mineral deposition was probed by X-ray diffraction. Cell viability analysis was performed using oral mucosa fibroblasts by: 1) quantifying the liberated deoxyribonucleic acid from dead cells, 2) detecting the amount of lactate dehydrogenase enzyme released by cells with damaged membranes, and 3) by examining the cytoplasmic esterase function and cell membranes integrity with a fluorescence-based method using the Live/Dead commercial kit. Data were analyzed by Kruskal-Wallis and Mann-Whitney tests. Precipitation of calcium and phosphate on the nanoparticles surfaces was observed in calcium-loaded nanoparticles. Non-loaded nanoparticles were found to be non-toxic in all the assays, calcium and zinc-loaded particles presented a dose dependent but very low cytotoxic effect. The ability of calcium-loaded nanoparticles to promote precipitation of calcium phosphate deposits, together with their observed non-toxicity may offer new strategies for periodontal disease treatment.
metadata.dc.identifier.doi: 10.1371/journal.pone.0166217
Appears in Collections:Producción 2020

Files in This Item:
File SizeFormat 
PMC5098795.pdf2,43 MBAdobe PDFView/Open

This item is protected by original copyright

This item is licensed under a Creative Commons License Creative Commons