Please use this identifier to cite or link to this item:
http://hdl.handle.net/10668/10832
Title: | Reference genes for gene expression studies in the mouse heart. |
Authors: | Ruiz-Villalba, Adrián Mattiotti, Andrea Gunst, Quinn D Cano-Ballesteros, Sara van den Hoff, Maurice J B Ruijter, Jan M |
metadata.dc.subject.mesh: | Animals Datasets as Topic Gene Expression Profiling Heart In Situ Hybridization Mice Myocardium Real-Time Polymerase Chain Reaction Reference Standards |
Issue Date: | 2-Feb-2017 |
Abstract: | To be accurate, quantitative Polymerase Chain Reaction (qPCR) studies require a set of stable reference genes for normalization. This is especially critical in cardiac research because of the diversity of the clinical and experimental conditions in the field. We analyzed the stability of previously described as potential reference genes in different subsets of cardiac tissues, each representing a different field in cardiac research. The qPCR dataset was based on 119 different tissue samples derived from cardiac development to pathology in mouse adult hearts. These samples were grouped into 47 tissue types. The stability of 9 candidate genes was analyzed in each of 12 experimental conditions comprising different groupings of these tissue types. Expression stability was determined with the geNorm module of qbase+. This analysis showed that different sets of two or three reference genes are required for analysis of qPCR data in different experimental conditions in murine cardiac research. |
URI: | http://hdl.handle.net/10668/10832 |
metadata.dc.identifier.doi: | 10.1038/s41598-017-00043-9 |
Appears in Collections: | Producción 2020 |
Files in This Item:
File | Size | Format | |
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PMC5428317.pdf | 2,12 MB | Adobe PDF | View/Open |
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