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Title: Poly(ethylmethacrylate-co-diethylaminoethyl acrylate) coating improves endothelial re-population, bio-mechanical and anti-thrombogenic properties of decellularized carotid arteries for blood vessel replacement.
Authors: López-Ruiz, Elena
Venkateswaran, Seshasailam
Perán, Macarena
Jiménez, Gema
Pernagallo, Salvatore
Díaz-Mochón, Juan J
Tura-Ceide, Olga
Arrebola, Francisco
Melchor, Juan
Soto, Juan
Rus, Guillermo
Real, Pedro J
Diaz-Ricart, María
Conde-González, Antonio
Bradley, Mark
Marchal, Juan A
metadata.dc.subject.mesh: Animals
Biocompatible Materials
Blood Platelets
Blood Vessel Prosthesis
Carotid Arteries
Carotid Artery Thrombosis
Endothelial Cells
Endothelium, Vascular
Human Umbilical Vein Endothelial Cells
Tissue Engineering
Tissue Scaffolds
Issue Date: 24-Mar-2017
Abstract: Decellularized vascular scaffolds are promising materials for vessel replacements. However, despite the natural origin of decellularized vessels, issues such as biomechanical incompatibility, immunogenicity risks and the hazards of thrombus formation, still need to be addressed. In this study, we coated decellularized vessels obtained from porcine carotid arteries with poly (ethylmethacrylate-co-diethylaminoethylacrylate) (8g7) with the purpose of improving endothelial coverage and minimizing platelet attachment while enhancing the mechanical properties of the decellularized vascular scaffolds. The polymer facilitated binding of endothelial cells (ECs) with high affinity and also induced endothelial cell capillary tube formation. In addition, platelets showed reduced adhesion on the polymer under flow conditions. Moreover, the coating of the decellularized arteries improved biomechanical properties by increasing its tensile strength and load. In addition, after 5 days in culture, ECs seeded on the luminal surface of 8g7-coated decellularized arteries showed good regeneration of the endothelium. Overall, this study shows that polymer coating of decellularized vessels provides a new strategy to improve re-endothelialization of vascular grafts, maintaining or enhancing mechanical properties while reducing the risk of thrombogenesis. These results could have potential applications in improving tissue-engineered vascular grafts for cardiovascular therapies with small caliber vessels.
metadata.dc.identifier.doi: 10.1038/s41598-017-00294-6
Appears in Collections:Producción 2020

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