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Title: Adamdec1, Ednrb and Ptgs1/Cox1, inflammation genes upregulated in the intestinal mucosa of obese rats, are downregulated by three probiotic strains.
Authors: Plaza-Díaz, Julio
Robles-Sánchez, Cándido
Abadía-Molina, Francisco
Morón-Calvente, Virginia
Sáez-Lara, María José
Ruiz-Bravo, Alfonso
Jiménez-Valera, María
Gil, Ángel
Gómez-Llorente, Carolina
Fontana, Luis
metadata.dc.subject.mesh: ADAM Proteins
Cyclooxygenase 1
Dendritic Cells
Gastrointestinal Microbiome
Gene Expression
Intestinal Mucosa
Membrane Proteins
Rats, Zucker
Receptor, Endothelin B
Issue Date: 16-May-2017
Abstract: We have previously reported that administration of Lactobacillus paracasei CNCM I-4034, Bifidobacterium breve CNCM I-4035 and Lactobacillus rhamnosus CNCM I-4036 to obese Zucker-Lepr fa/fa rats attenuates liver steatosis and exerts anti-inflammatory effects. The goal of the present work was to investigate the modulation of gene expression in intestinal mucosa samples of obese Zucker-Lepr fa/fa rats fed the probiotic strains using a DNA microarray and postgenomic techniques. We also measured secretory IgA content in the gut and lipopolysaccharide (LPS)-binding protein (LBP) in serum. Expression of three genes (Adamdec1, Ednrb and Ptgs1/Cox1) was up-regulated in the intestinal mucosa of the obese rats compared with that in the rats when they were still lean. Probiotic administration down-regulated expression of Adamdec1 and Ednrb at the mRNA and protein levels and that of Ptgs1/Cox1 at the mRNA level, and this effect was in part mediated by a decrease in both macrophage and dendritic cell populations. Probiotic treatment also increased secretory IgA content and diminished the LBP concentration. Based on results reported in this work and else where, we propose a possible mechanism of action for these bacterial strains.
metadata.dc.identifier.doi: 10.1038/s41598-017-02203-3
Appears in Collections:Producción 2020

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