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Title: Tracking cell proliferation using a nanotechnology-based approach.
Authors: Altea-Manzano, Patricia
Unciti-Broceta, Juan Diego
Cano-Cortes, Victoria
Ruiz-Blas, María Paz
Valero-Griñan, Teresa
Diaz-Mochon, Juan Jose
Sanchez-Martin, Rosario
Keywords: cell proliferation;cell tracking;flow cytometry;fluorescent nanoparticles;nanofection
metadata.dc.subject.mesh: Cell Cycle
Cell Line, Tumor
Cell Proliferation
Cell Tracking
Feasibility Studies
Flow Cytometry
Fluorescent Dyes
Leukocytes, Mononuclear
Reproducibility of Results
Staining and Labeling
Surface Properties
Issue Date: 17-May-2017
Abstract: To develop an efficient nanotechnology fluorescence-based method to track cell proliferation to avoid the limitations of current cell-labeling dyes. Synthesis, PEGylation, bifunctionalization and labeling with a fluorophore (Cy5) of 200 nm polystyrene nanoparticles (NPs) were performed. These NPs were characterized and assessed for in vitro long-term monitoring of cell proliferation. The optimization and validation of this method to track long-term cell proliferation assays have been achieved with high reproducibility, without cell cycle disruption. This method has been successfully applied in several adherent and suspension cells including hard-to-transfect cells and isolated human primary lymphocytes. A novel approach to track efficiently cellular proliferation by flow cytometry using fluorescence labeled NPs has been successfully developed. [Formula: see text].
metadata.dc.identifier.doi: 10.2217/nnm-2017-0118
Appears in Collections:Producción 2020

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