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Title: Survivin, a key player in cancer progression, increases in obesity and protects adipose tissue stem cells from apoptosis.
Authors: Ejarque, Miriam
Ceperuelo-Mallafré, Victòria
Serena, Carolina
Pachón, Gisela
Núñez-Álvarez, Yaiza
Terrón-Puig, Margarida
Calvo, Enrique
Núñez-Roa, Catalina
Oliva-Olivera, Wilfredo
Tinahones, Francisco J
Peinado, Miguel Angel
Vendrell, Joan
Fernández-Veledo, Sonia
metadata.dc.subject.mesh: Adipose Tissue
Disease Progression
Epigenesis, Genetic
Inhibitor of Apoptosis Proteins
Middle Aged
Protein Biosynthesis
Stem Cells
Transcription, Genetic
Issue Date: 18-May-2017
Abstract: Adipose tissue (AT) has a central role in obesity-related metabolic imbalance through the dysregulated production of cytokines and adipokines. In addition to its known risk for cardiovascular disease and diabetes, obesity is also a major risk for cancer. We investigated the impact of obesity for the expression of survivin, an antiapoptotic protein upregulated by adipokines and a diagnostic biomarker of tumor onset and recurrence. In a cross-sectional study of 111 subjects classified by body mass index, circulating levels of survivin and gene expression in subcutaneous AT were significantly higher in obese patients and positively correlated with leptin. Within AT, survivin was primarily detected in human adipocyte-derived stem cells (hASCs), the adipocyte precursors that determine AT expansion. Remarkably, survivin expression was significantly higher in hASCs isolated from obese patients that from lean controls and was increased by proinflammatory M1 macrophage soluble factors including IL-1β. Analysis of survivin expression in hASCs revealed a complex regulation including epigenetic modifications and protein stability. Surprisingly, obese hASCs showed survivin promoter hypermethylation that correlated with a significant decrease in its mRNA levels. Nonetheless, a lower level of mir-203, which inhibits survivin protein translation, and higher protein stability, was found in obese hASCs compared with their lean counterparts. We discovered that survivin levels determine the susceptibility of hASCs to apoptotic stimuli (including leptin and hypoxia). Accordingly, hASCs from an obese setting were protected from apoptosis. Collectively, these data shed new light on the molecular mechanisms governing AT expansion in obesity through promotion of hASCs that are resistant to apoptosis, and point to survivin as a potential new molecular player in the communication between AT and tumor cells. Thus, inhibition of apoptosis targeting survivin might represent an effective strategy for both obesity and cancer therapy.
metadata.dc.identifier.doi: 10.1038/cddis.2017.209
Appears in Collections:Producción 2020

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