Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/11406
Title: Diversity of Pneumocystis jirovecii Across Europe: A Multicentre Observational Study.
Authors: Alanio, Alexandre
Gits-Muselli, Maud
Guigue, Nicolas
Desnos-Ollivier, Marie
Calderon, Enrique J
Di Cave, David
Dupont, Damien
Hamprecht, Axel
Hauser, Philippe M
Helweg-Larsen, Jannik
Kicia, Marta
Lagrou, Katrien
Lengerova, Martina
Matos, Olga
Melchers, Willem J G
Morio, Florent
Nevez, Gilles
Totet, Anne
White, Lewis P
Bretagne, Stéphane
Keywords: Europe;Genotyping;MLS typing;Microsatellites;Mixed infection;Pneumocystis jirovecii;Transmission
metadata.dc.subject.mesh: Adult
Aged
DNA, Fungal
Europe
Female
Genetic Variation
Genotyping Techniques
Humans
Male
Microsatellite Repeats
Middle Aged
Phylogeny
Phylogeography
Pneumocystis carinii
Pneumonia, Pneumocystis
Issue Date: 29-Jun-2017
Abstract: Pneumocystis jirovecii is an airborne human-specific ascomycetous fungus responsible for Pneumocystis pneumonia (PCP) in immunocompromised patients, affecting >500,000 patients per year (www.gaffi.org). The understanding of its epidemiology is limited by the lack of standardised culture. Recent genotyping data suggests a limited genetic diversity of P. jirovecii. The objective of the study was to assess the diversity of P. jirovecii across European hospitals and analyse P. jirovecii diversity in respect to clinical data obtained from the patients. Genotyping was performed using six already validated short tandem repeat (STR) markers on 249 samples (median: 17 per centre interquartile range [11-20]) from PCP patients of 16 European centres. Mixtures of STR markers (i.e., ≥2 alleles for ≥1 locus) were detected in 67.6% (interquartile range [61.4; 76.5]) of the samples. Mixture was significantly associated with the underlying disease of the patient, with an increased proportion in HIV patients (78.3%) and a decreased proportion in renal transplant recipients (33.3%) (p500,000 patients per year (www.gaffi.org). The understanding of its epidemiology is limited by the lack of standardised culture. Recent genotyping data suggests a limited genetic diversity of P. jirovecii. The objective of the study was to assess the diversity of P. jirovecii across European hospitals and analyse P. jirovecii diversity in respect to clinical data obtained from the patients. Genotyping was performed using six already validated short tandem repeat (STR) markers on 249 samples (median: 17 per centre interquartile range [11-20]) from PCP patients of 16 European centres. Mixtures of STR markers (i.e., ≥2 alleles for ≥1 locus) were detected in 67.6% (interquartile range [61.4; 76.5]) of the samples. Mixture was significantly associated with the underlying disease of the patient, with an increased proportion in HIV patients (78.3%) and a decreased proportion in renal transplant recipients (33.3%) (p
URI: http://hdl.handle.net/10668/11406
metadata.dc.identifier.doi: 10.1016/j.ebiom.2017.06.027
Appears in Collections:Producción 2020

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