Please use this identifier to cite or link to this item:
http://hdl.handle.net/10668/11406
Title: | Diversity of Pneumocystis jirovecii Across Europe: A Multicentre Observational Study. |
Authors: | Alanio, Alexandre Gits-Muselli, Maud Guigue, Nicolas Desnos-Ollivier, Marie Calderon, Enrique J Di Cave, David Dupont, Damien Hamprecht, Axel Hauser, Philippe M Helweg-Larsen, Jannik Kicia, Marta Lagrou, Katrien Lengerova, Martina Matos, Olga Melchers, Willem J G Morio, Florent Nevez, Gilles Totet, Anne White, Lewis P Bretagne, Stéphane |
Keywords: | Europe;Genotyping;MLS typing;Microsatellites;Mixed infection;Pneumocystis jirovecii;Transmission |
metadata.dc.subject.mesh: | Adult Aged DNA, Fungal Europe Female Genetic Variation Genotyping Techniques Humans Male Microsatellite Repeats Middle Aged Phylogeny Phylogeography Pneumocystis carinii Pneumonia, Pneumocystis |
Issue Date: | 29-Jun-2017 |
Abstract: | Pneumocystis jirovecii is an airborne human-specific ascomycetous fungus responsible for Pneumocystis pneumonia (PCP) in immunocompromised patients, affecting >500,000 patients per year (www.gaffi.org). The understanding of its epidemiology is limited by the lack of standardised culture. Recent genotyping data suggests a limited genetic diversity of P. jirovecii. The objective of the study was to assess the diversity of P. jirovecii across European hospitals and analyse P. jirovecii diversity in respect to clinical data obtained from the patients. Genotyping was performed using six already validated short tandem repeat (STR) markers on 249 samples (median: 17 per centre interquartile range [11-20]) from PCP patients of 16 European centres. Mixtures of STR markers (i.e., ≥2 alleles for ≥1 locus) were detected in 67.6% (interquartile range [61.4; 76.5]) of the samples. Mixture was significantly associated with the underlying disease of the patient, with an increased proportion in HIV patients (78.3%) and a decreased proportion in renal transplant recipients (33.3%) (p500,000 patients per year (www.gaffi.org). The understanding of its epidemiology is limited by the lack of standardised culture. Recent genotyping data suggests a limited genetic diversity of P. jirovecii. The objective of the study was to assess the diversity of P. jirovecii across European hospitals and analyse P. jirovecii diversity in respect to clinical data obtained from the patients. Genotyping was performed using six already validated short tandem repeat (STR) markers on 249 samples (median: 17 per centre interquartile range [11-20]) from PCP patients of 16 European centres. Mixtures of STR markers (i.e., ≥2 alleles for ≥1 locus) were detected in 67.6% (interquartile range [61.4; 76.5]) of the samples. Mixture was significantly associated with the underlying disease of the patient, with an increased proportion in HIV patients (78.3%) and a decreased proportion in renal transplant recipients (33.3%) (p |
URI: | http://hdl.handle.net/10668/11406 |
metadata.dc.identifier.doi: | 10.1016/j.ebiom.2017.06.027 |
Appears in Collections: | Producción 2020 |
Files in This Item:
File | Size | Format | |
---|---|---|---|
PMC5552205.pdf | 1,04 MB | Adobe PDF | View/Open |
This item is protected by original copyright |
This item is licensed under a Creative Commons License