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Title: Magnesium Chloride promotes Osteogenesis through Notch signaling activation and expansion of Mesenchymal Stem Cells.
Authors: Díaz-Tocados, Juan M
Herencia, Carmen
Martínez-Moreno, Julio M
Montes de Oca, Addy
Rodríguez-Ortiz, Maria E
Vergara, Noemi
Blanco, Alfonso
Steppan, Sonja
Almadén, Yolanda
Rodríguez, Mariano
Muñoz-Castañeda, Juan R
metadata.dc.subject.mesh: Animals
Bone and Bones
Boron Compounds
Cell Differentiation
Cell Proliferation
Cells, Cultured
Enzyme Inhibitors
Magnesium Chloride
Mesenchymal Stem Cells
Microscopy, Electron
Receptors, Notch
Signal Transduction
TRPM Cation Channels
Issue Date: 10-Aug-2017
Abstract: Mesenchymal stem cells (MSC) are osteoblasts progenitors and a variety of studies suggest that they may play an important role for the health in the field of bone regeneration. Magnesium supplementation is gaining importance as adjuvant treatment to improve osteogenesis, although the mechanisms involving this process are not well understood. The objective of this study was to investigate the effects of magnesium on MSC differentiation. Here we show that in rat bone marrow MSC, magnesium chloride increases MSC proliferation in a dose-dependent manner promoting osteogenic differentiation and mineralization. These effects are reduced by 2-APB administration, an inhibitor of magnesium channel TRPM7. Of note, magnesium supplementation did not increase the canonical Wnt/β-catenin pathway, although it promoted the activation of Notch1 signaling, which was also decreased by addition of 2-APB. Electron microscopy showed higher proliferation, organization and maturation of osteoblasts in bone decellularized scaffolds after magnesium addition. In summary, our results demonstrate that magnesium chloride enhances MSC proliferation by Notch1 signaling activation and induces osteogenic differentiation, shedding light on the understanding of the role of magnesium during bone regeneration.
metadata.dc.identifier.doi: 10.1038/s41598-017-08379-y
Appears in Collections:Producción 2020

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