Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/1157
Título : Rab18 dynamics in adipocytes in relation to lipogenesis, lipolysis and obesity.
Autor : Pulido, Marina R
Díaz-Ruiz, Alberto
Jiménez-Gómez, Yolanda
García-Navarro, Socorro
Gracia-Navarro, Francisco
Tinahones, Francisco
López-Miranda, José
Frühbeck, Gema
Vázquez-Martínez, Rafael
Malagón, María M
Filiación: [Pulido,MR; Díaz-Ruiz,A; Jiménez-Gómez,Y; García-Navarro,S; Gracia-Navarro,F; Vázquez-Martínez,R; Malagón,MM] Department of Cell Biology, Physiology and Immunology, University of Córdoba, Córdoba, Spain. [Pulido,MR; Díaz-Ruiz,A; Jiménez-Gómez,Y; García-Navarro,S; Tinahones,F; López-Miranda,J; Frühbeck,G; Vázquez-Martínez,R; Malagón,MM] Centro de Investigación Biomédica en Red Fisiopatología de la Obesidad y Nutrición, Córdoba, Spain. [Pulido,MR; Díaz-Ruiz,A; Jiménez-Gómez,Y; García-Navarro,S; Gracia-Navarro,F; López-Miranda,J; Vázquez-Martínez,R; Malagón,MM] Instituto Maimónides de Investigación Biomédica (IMIBIC), Córdoba, Spain. [Tinahones,J] Servicio de Endocrinología y Nutrición, Hospital Clínico Virgen de la Victoria, Málaga, Spain. [López-Miranda,J] Lipids and Atherosclerosis Unit, Hospital Universitario Reina Sofía, Córdoba, Spain. [Frühbeck,G] Metabolic Research Laboratory, University of Navarra, Pamplona, Spain.
Palabras clave : Rab18 protein, mouse
Adipocitos
Adiponectina
Western Blotting
Células Cultivadas
Retículo Endoplásmico
Hipoglucemiantes
Técnicas para Inmunoenzimas
Metabolismo de los Lípidos
Obesidad
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
Células 3T3-L1
Insulina
MeSH: Medical Subject Headings::Anatomy::Cells::Connective Tissue Cells::Adipocytes
Medical Subject Headings::Chemicals and Drugs::Hormones, Hormone Substitutes, and Hormone Antagonists::Hormones::Peptide Hormones::Adipokines::Adiponectin
Medical Subject Headings::Organisms::Eukaryota::Animals
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Electrophoresis::Blotting, Western
Medical Subject Headings::Anatomy::Cells::Cells, Cultured
Medical Subject Headings::Anatomy::Cells::Cellular Structures::Intracellular Space::Cytoplasm::Cytoplasmic Structures::Organelles::Endoplasmic Reticulum
Medical Subject Headings::Check Tags::Female
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Physiological Effects of Drugs::Hypoglycemic Agents
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Immunologic Techniques::Immunoassay::Immunoenzyme Techniques
Medical Subject Headings::Chemicals and Drugs::Hormones, Hormone Substitutes, and Hormone Antagonists::Hormones::Peptide Hormones::Pancreatic Hormones::Insulins
Medical Subject Headings::Phenomena and Processes::Metabolic Phenomena::Metabolism::Lipid Metabolism
Medical Subject Headings::Phenomena and Processes::Metabolic Phenomena::Metabolism::Lipid Metabolism::Lipogenesis
Medical Subject Headings::Phenomena and Processes::Metabolic Phenomena::Metabolism::Lipid Metabolism::Lipolysis
Medical Subject Headings::Check Tags::Male
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Mice
Medical Subject Headings::Diseases::Nutritional and Metabolic Diseases::Nutrition Disorders::Overnutrition::Obesity
Medical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::RNA::RNA, Messenger
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Nucleic Acid Amplification Techniques::Polymerase Chain Reaction::Reverse Transcriptase Polymerase Chain Reaction
Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Hydrolases::Acid Anhydride Hydrolases::GTP Phosphohydrolases::GTP-Binding Proteins
Medical Subject Headings::Anatomy::Cells::Cells, Cultured::Cell Line::3T3 Cells
Fecha de publicación : 28-Jul-2011
Editorial : Public Library of Science
Cita Bibliográfica: Pulido MR, Diaz-Ruiz A, Jiménez-Gómez Y, Garcia-Navarro S, Gracia-Navarro F, Tinahones F, et al. Rab18 dynamics in adipocytes in relation to lipogenesis, lipolysis and obesity. PLoS ONE. 2011; 6(7):e22931
Abstract: Lipid droplets (LDs) are organelles that coordinate lipid storage and mobilization, both processes being especially important in cells specialized in managing fat, the adipocytes. Proteomic analyses of LDs have consistently identified the small GTPase Rab18 as a component of the LD coat. However, the specific contribution of Rab18 to adipocyte function remains to be elucidated. Herein, we have analyzed Rab18 expression, intracellular localization and function in relation to the metabolic status of adipocytes. We show that Rab18 production increases during adipogenic differentiation of 3T3-L1 cells. In addition, our data show that insulin induces, via phosphatidylinositol 3-kinase (PI3K), the recruitment of Rab18 to the surface of LDs. Furthermore, Rab18 overexpression increased basal lipogenesis and Rab18 silencing impaired the lipogenic response to insulin, thereby suggesting that this GTPase promotes fat accumulation in adipocytes. On the other hand, studies of the β-adrenergic receptor agonist isoproterenol confirmed and extended previous evidence for the participation of Rab18 in lipolysis. Together, our data support the view that Rab18 is a common mediator of lipolysis and lipogenesis and suggests that the endoplasmic reticulum (ER) is the link that enables Rab18 action on these two processes. Finally, we describe, for the first time, the presence of Rab18 in human adipose tissue, wherein the expression of this GTPase exhibits sex- and depot-specific differences and is correlated to obesity. Taken together, these findings indicate that Rab18 is involved in insulin-mediated lipogenesis, as well as in β-adrenergic-induced lipolysis, likely facilitating interaction of LDs with ER membranes and the exchange of lipids between these compartments. A role for Rab18 in the regulation of adipocyte biology under both normal and pathological conditions is proposed.
Descripción : Journal Article; Research Support, Non-U.S. Gov't;
URI: http://hdl.handle.net/10668/1157
Versión del editor : http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0022931
DOI: 10.1371/journal.pone.0022931
ISSN : 1932-6203 (Online)
Appears in Collections:01- Artículos - IMIBIC. Instituto Maimónides de Investigación Biomédica de Córdoba
01- Artículos - Hospital Virgen de la Victoria
01- Artículos - Hospital Reina Sofía

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