Please use this identifier to cite or link to this item:
Title: Intestinal anti-inflammatory effects of goat whey on DNBS-induced colitis in mice.
Authors: Araújo, Daline F S
Guerra, Gerlane C B
Pintado, Maria Manuela E
Sousa, Yasmim R F
Algieri, Francesca
Rodriguez-Nogales, Alba
Araújo, Raimundo F
Gálvez, Julio
Queiroga, Rita de Cássia R E
Rodriguez-Cabezas, Maria Elena
metadata.dc.subject.mesh: Animals
Anti-Inflammatory Agents
Disease Models, Animal
Enzyme-Linked Immunosorbent Assay
Fluorescent Antibody Technique
Gene Expression Regulation
Inflammation Mediators
Intestinal Mucosa
RAW 264.7 Cells
Real-Time Polymerase Chain Reaction
Issue Date: 28-Sep-2017
Abstract: This study evaluated the intestinal anti-inflammatory effects of goat whey in a mouse model of colitis induced by 2,4-dinitrobenzenesulfonic acid that resembles human IBD. At a concentration of 4 g/kg/day, the goat whey improved the symptoms of intestinal inflammation, namely by decreasing the disease activity index, colonic weight/length, and leukocyte infiltration. Moreover, goat whey inhibited NF-κB p65 and p38 MAPK signaling pathways and consequently down-regulated the gene expression of various proinflammatory markers such as IL-1β, IL-6, IL-17, TNF-α, iNOS, MMP-9, ICAM-1. Also, goat whey increased the expression of proteins such as mucins, occludin proteins and cytokine signalling suppressors. The immunomodulatory properties of goat whey were also evaluated in vitro using the murine macrophage cell line Raw 264 and CMT-93 cells derived from mouse rectum carcinomas. The results revealed the ability of goat whey to inhibit the production of NO and reduce IL-6 production in LPS-stimulated cells. In conclusion, goat whey exhibited anti-inflammatory effects in the DNBS model of intestinal inflammation, and these observations were confirmed by its immunomodulatory properties in vitro. Together, our results indicate that goat whey could have applications for the treatment of IBD.
metadata.dc.identifier.doi: 10.1371/journal.pone.0185382
Appears in Collections:Producción 2020

Files in This Item:
File SizeFormat 
PMC5619769.pdf7,86 MBAdobe PDFView/Open

This item is protected by original copyright

This item is licensed under a Creative Commons License Creative Commons