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Title: Late rDNA Condensation Ensures Timely Cdc14 Release and Coordination of Mitotic Exit Signaling with Nucleolar Segregation.
Authors: de Los Santos-Velázquez, Ana Isabel
de Oya, Inés G
Manzano-López, Javier
Monje-Casas, Fernando
Keywords: Cdc14;FEAR;MEN;condensin;mitotic exit;nucleolus;snoRNPs
metadata.dc.subject.mesh: Cell Cycle Proteins
Cytoskeletal Proteins
DNA, Fungal
DNA, Ribosomal
DNA-Directed RNA Polymerases
Gene Deletion
Guanine Nucleotide Exchange Factors
Nucleolus Organizer Region
Protein Tyrosine Phosphatases
Ribonucleoproteins, Small Nucleolar
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Issue Date: 19-Oct-2017
Abstract: The nucleolus plays a pivotal role in multiple key cellular processes. An illustrative example is the regulation of mitotic exit in Saccharomyces cerevisiae through the nucleolar sequestration of the Cdc14 phosphatase. The peculiar structure of the nucleolus, however, has also its drawbacks. The repetitive nature of the rDNA gives rise to cohesion-independent linkages whose resolution in budding yeast requires the Cdc14-dependent inhibition of rRNA transcription, which facilitates condensin accessibility to this locus. Thus, the rDNA condenses and segregates later than most other yeast genomic regions. Here, we show that defective function of a small nucleolar ribonucleoprotein particle (snoRNP) assembly factor facilitates condensin accessibility to the rDNA and induces nucleolar hyper-condensation. Interestingly, this increased compaction of the nucleolus interferes with the proper release of Cdc14 from this organelle. This observation provides an explanation for the delayed rDNA condensation in budding yeast, which is necessary to efficiently coordinate timely Cdc14 release and mitotic exit with nucleolar compaction and segregation.
metadata.dc.identifier.doi: 10.1016/j.cub.2017.09.028
Appears in Collections:Producción 2020

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