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Title: Human Pluripotent Stem Cell Differentiation into Functional Epicardial Progenitor Cells.
Authors: Guadix, Juan Antonio
Orlova, Valeria V
Giacomelli, Elisa
Bellin, Milena
Ribeiro, Marcelo C
Mummery, Christine L
Pérez-Pomares, José M
Passier, Robert
Keywords: cardiovascular;differentiation;human pluripotent stem cells;proepicardium;progenitor cells
metadata.dc.subject.mesh: Animals
Bone Morphogenetic Protein 4
Cardiovascular System
Cell Adhesion
Cell Culture Techniques
Cell Differentiation
Chick Embryo
Embryonic Development
Gene Expression Regulation, Developmental
Induced Pluripotent Stem Cells
Myocytes, Cardiac
Receptor, Platelet-Derived Growth Factor alpha
Stem Cells
Issue Date: 22-Nov-2017
Abstract: Human pluripotent stem cells (hPSCs) are widely used to study cardiovascular cell differentiation and function. Here, we induced differentiation of hPSCs (both embryonic and induced) to proepicardial/epicardial progenitor cells that cover the heart during development. Addition of retinoic acid (RA) and bone morphogenetic protein 4 (BMP4) promoted expression of the mesodermal marker PDGFRα, upregulated characteristic (pro)epicardial progenitor cell genes, and downregulated transcription of myocardial genes. We confirmed the (pro)epicardial-like properties of these cells using in vitro co-culture assays and in ovo grafting of hPSC-epicardial cells into chick embryos. Our data show that RA + BMP4-treated hPSCs differentiate into (pro)epicardial-like cells displaying functional properties (adhesion and spreading over the myocardium) of their in vivo counterpart. The results extend evidence that hPSCs are an excellent model to study (pro)epicardial differentiation into cardiovascular cells in human development and evaluate their potential for cardiac regeneration.
metadata.dc.identifier.doi: 10.1016/j.stemcr.2017.10.023
Appears in Collections:Producción 2020

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