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Title: Evolution of Energy Related Metabolites in Plasma from Newborns with Hypoxic-Ischemic Encephalopathy during Hypothermia Treatment.
Authors: Sánchez-Illana, Ángel
Núñez-Ramiro, Antonio
Cernada, María
Parra-Llorca, Anna
Valverde, Eva
Blanco, Dorotea
Moral-Pumarega, Maria Teresa
Cabañas, Fernando
Boix, Hector
Pavon, Antonio
Chaffanel, Mercedes
Benavente-Fernández, Isabel
Tofe, Inés
Loureiro, Begoña
Fernández-Lorenzo, José R
Fernández-Colomer, Belén
García-Robles, Ana
Kuligowski, Julia
Vento, Máximo
HYPOTOP Study Group
metadata.dc.subject.mesh: 3-Hydroxybutyric Acid
Case-Control Studies
Gas Chromatography-Mass Spectrometry
Hypothermia, Induced
Hypoxia-Ischemia, Brain
Infant, Newborn
Ketone Bodies
Lactic Acid
Limit of Detection
Pyruvic Acid
Issue Date: 6-Dec-2017
Abstract: Therapeutic hypothermia (TH) initiated within 6 h from birth is the most effective therapeutic approach for moderate to severe hypoxic-ischemic encephalopathy (HIE). However, underlying mechanisms and effects on the human metabolism are not yet fully understood. This work aims at studying the evolution of several energy related key metabolites in newborns with HIE undergoing TH employing gas chromatography - mass spectrometry. The method was validated following stringent FDA requirements and applied to 194 samples from a subgroup of newborns with HIE (N = 61) enrolled in a multicenter clinical trial (HYPOTOP) for the determination of lactate, pyruvate, ketone bodies and several Krebs cycle metabolites at different sampling time points. The analysis of plasma samples from newborns with HIE revealed a decrease of lactate, pyruvate and β-hydroxybutyrate concentrations, whereas rising malate concentrations were observed. In healthy control newborns (N = 19) significantly lower levels of pyruvate and lactate were found in comparison to age-matched newborns with HIE undergoing TH, whereas acetoacetate and β-hydroxybutyrate levels were clearly increased. Access to a validated analytical method and a controlled cohort of newborns with HIE undergoing hypothermia treatment for the first time allowed the in-depth study of the evolution of key metabolites of metabolic junctions in this special population.
metadata.dc.identifier.doi: 10.1038/s41598-017-17202-7
Appears in Collections:Producción 2020

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