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Title: Clinoptilolite in Dextran Sulphate Sodium-Induced Murine Colitis: Efficacy and Safety of a Microparticulate Preparation.
Authors: Nizet, Stéphane
Muñoz, Eduardo
Fiebich, Bernd L
Abuja, Peter M
Kashofer, Karl
Zatloukal, Kurt
Tangermann, Simone
Kenner, Lukas
Tschegg, Cornelius
Nagl, Dietmar
Scheichl, Laurenz
Meisslitzer-Ruppitsch, Claudia
Freissmuth, Michael
Berger, Thomas
Keywords: DSS;colitis;inflammation;zeolite
metadata.dc.subject.mesh: Animals
Dextran Sulfate
Disease Models, Animal
Drug Compounding
Gastrointestinal Microbiome
Intestinal Diseases
Mice, Inbred C57BL
Issue Date: 2017
Abstract: Clinoptilolite is an aluminium silicate of natural origin; the microporous structure and the net negative charge of its crystal lattice allows for adsorption of ions, toxins, inflammatory mediators, and some microorganisms. We generated 2 preparations of purified clinoptilolite, which differed by about 10-fold in particle size, ie, a standard powder (GHC1) and a microparticulate fraction (GHC2) with a size of 3.6 µm and 0.39 µm (d50) respectively. These were examined for their ability to accelerate the recovery of mice from DSS (dextran sulphate sodium)-induced intestinal inflammation. Efficacy of clinoptilolite preparations was investigated by administering DSS-treated mice twice daily with 30 mg GHC2 or GHC1 for 5 consecutive days, followed by 5 days of recovery without DSS. To explore the safety of the microparticulate preparation (GHC2), mice were subjected to 4 cycles of DSS-exposure. We specifically verified that clinoptilolite microparticles were not systemically bioavailable by examining the gut tissue and the liver for the accumulation of microparticles by transmission electron microscopy. Treatment of mice with GHC2 was superior to GHC1 and as effective as the reference compound 5-aminosalicylic acid in ameliorating the damage induced by the exposure to DSS. In addition, no clinoptilolite particle was observed in the intestinal epithelial layer, gut-associated lymph follicles, or in the liver. Our observations confirm that a microparticulate preparation of clinoptilolite is safe and effective in a murine model of inflammatory bowel disease and supports the hypothesis that the adsorptive capacity of clinoptilolite is of potential therapeutic relevance.
metadata.dc.identifier.doi: 10.1093/ibd/izx042
Appears in Collections:Producción 2020

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