Please use this identifier to cite or link to this item:
Title: Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness: A Method to Direct Chondrogenic Differentiation.
Authors: Casanellas, Ignasi
Lagunas, Anna
Tsintzou, Iro
Vida, Yolanda
Collado, Daniel
Pérez-Inestrosa, Ezequiel
Rodríguez-Pereira, Cristina
Magalhaes, Joana
Gorostiza, Pau
Andrades, José A
Becerra, José
Samitier, Josep
metadata.dc.subject.mesh: Adhesiveness
Cell Adhesion
Mesenchymal Stem Cells
NIH 3T3 Cells
Surface Properties
Issue Date: 20-Jan-2018
Abstract: Cellular adhesion and differentiation is conditioned by the nanoscale disposition of the extracellular matrix (ECM) components, with local concentrations having a major effect. Here we present a method to obtain large-scale uneven nanopatterns of arginine-glycine-aspartic acid (RGD)-functionalized dendrimers that permit the nanoscale control of local RGD surface density. Nanopatterns are formed by surface adsorption of dendrimers from solutions at different initial concentrations and are characterized by water contact angle (CA), X-ray photoelectron spectroscopy (XPS), and scanning probe microscopy techniques such as scanning tunneling microscopy (STM) and atomic force microscopy (AFM). The local surface density of RGD is measured using AFM images by means of probability contour maps of minimum interparticle distances and then correlated with cell adhesion response and differentiation. The nanopatterning method presented here is a simple procedure that can be scaled up in a straightforward manner to large surface areas. It is thus fully compatible with cell culture protocols and can be applied to other ligands that exert concentration-dependent effects on cells.
metadata.dc.identifier.doi: 10.3791/56347
Appears in Collections:Producción 2020

Files in This Item:
There are no files associated with this item.

This item is protected by original copyright

Except where otherwise noted, Items on the Andalusian Health Repository site are licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives License.