Please use this identifier to cite or link to this item:
Title: Natural Extracts Abolished Lipid Accumulation in Cells Harbouring non-favourable PNPLA3 genotype.
Authors: Rojas, Ángela
Gallego, Paloma
Gil-Gómez, Antonio
Muñoz-Hernández, Rocío
Rojas, Lourdes
Maldonado, Rosario
Gallego, Rocío
García-Valdecasas, Marta
Del Campo, José A
Bautista, Juan D
Romero-Gómez, Manuel
Keywords: Steatosis. Lipid droplets. Triglycerides. Quercetin. Cynara scolymus. Agaricus Bisporus. Mushroom. Artichoke.
metadata.dc.subject.mesh: Acetyl-CoA C-Acetyltransferase
Cell Line, Tumor
Cynara scolymus
Membrane Proteins
Non-alcoholic Fatty Liver Disease
Oleic Acid
PPAR alpha
PPAR gamma
Plant Extracts
Sterol Regulatory Element Binding Protein 1
Issue Date: 2018
Abstract: Background & aims. G-allele of PNPLA3 (rs738409) favours triglycerides accumulation and steatosis. In this study, we examined the effect of quercetin and natural extracts from mushroom and artichoke on reducing lipid accumulation in hepatic cells. Huh7.5 cells were exposed to oleic acid (OA) and treated with quercetin and extracts to observe the lipid accumulation, the intracellular-TG concentration and the LD size. Sterol regulatory element binding proteins-1 (SREBP-1), peroxisome proliferator-activated receptor (PPARα-γ) and cholesterol acyltransferase (ACAT) gene expression levels were analysed. Quercetin decreased the intracellular lipids, LD size and the levels of intracellular-TG through the down-regulation of SREBP-1c, PPARγ and ACAT1 increasing PPARα. The natural-extracts suppressed OA-induced lipid accumulation and the intracellular-TG. They down-regulate the hepatic lipogenesis through SREBP-1c, besides the activation of lipolysis through the increasing of PPARα expression. Quercetin and the aqueous extracts decrease intracellular lipid accumulation by down-regulation of lipogenesis and up-regulation of lipolysis.
metadata.dc.identifier.doi: 10.5604/01.3001.0010.8642
ISSN: 1665-2681
Appears in Collections:Producción 2020

Files in This Item:
There are no files associated with this item.

This item is protected by original copyright

This item is licensed under a Creative Commons License Creative Commons