Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/1409
Título : The involvement of thaumatin-like proteins in plant food cross-reactivity: a multicenter study using a specific protein microarray
Autor : Palacín, Arantxa
Rivas, Luis A.
Gómez-Casado, Cristina
Aguirre, Jacobo
Tordesillas, Leticia
Bartra, Joan
Blanco, Carlos
Carrillo, Teresa
Cuesta-Herranz, Javier
Cumplido Bonny, José A.
Flores, Enrique
García-Alvarez-Eire, Mar G.
García-Nuñez, Ignacio
Fernández, Francisco J.
Gamboa, Pedro
Muñoz, Rosa
Sánchez-Monge, Rosa
Torres, Maria
Varela Losada, Susana
Villalba, Mayte
Vega, Francisco
Parro, Victor
Blanca, Miguel
Salcedo, Gabriel
Díaz-Perales, Araceli
Filiación: [Palacín,A; Gómez-Casado,C; Tordesillas,L; Sánchez-Monge,R; Díaz-Perales,A] Centro de Biotecnología y Genómica de Plantas, Campus de Montegancedo, Pozuelo de Alarcón, Madrid, Spain. [Rivas,LA; Aguirre,J; Parro,V] Departamento de Evolución Molecular, Centro de Astrobiología (INTA-CSIC), Torrejón de Ardoz, Madrid, Spain. [Bartra,J; Muñoz,R] Unitat d’Allèrgia, Servei Pneumologia i Allèrgia Respiratòria, Hospital Clínic, Universitat de Barcelona, Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Centro de Investigaciones Biomédicas en Red de Enfermedades Respiratorias (CIBERES), Barcelona, Spain. [Blanco,C; Vega,F] Servicio de Alergia, Hospital Universitario de la Princesa, Instituto de Investigación Sanitaria Princesa (IP), Madrid, Spain. [Carrillo,T; Cumplido Bonny,JA] Servicio de Alergología, Hospital Universitario de Gran Canaria Dr. Negrín, Las Palmas de Gran Canaria, Spain. [Cuesta-Herranz,J] Servicio de Alergia, Fundación Jiménez Díaz, Madrid, Spain. [Flores,E; Fernández,FJ] Unidad de Alergia, Hospital General Universitario, Alicante, Spain. [García-Alvarez-Eire,MG; Varela Losada,S] Unidad de Alergología, Complexo Hospitalario, Ourense, Spain. [García-Nuñez,I; Blanca,M] Laboratorio de Investigación, Fundación IMABIS-Carlos Haya Hospital, Hospital Civil, Málaga, Spain. [Gamboa,P] Servicio de Alergia, Hospital de Basurto, Bilbao, Spain. [Torres,M; Villalba,M] Departamento de Bioquímica y Biología Molecular I, Facultad de Químicas, Universidad Complutense, Madrid, Spain. [Salcedo,G]Departamento de Biotecnología, ETSI Agrónomos, Universidad Politécnica, Madrid, Spain.
Palabras clave : España
Geografía
Reacciones cruzadas
Hipersensibilidad a los alimentos
Inmunización
Inmunoensayo
Inmunoglobulina E
Peso molecular
Proteínas de plantas
Polen
Análisis por matrices de proteínas
MeSH: Medical Subject Headings::Named Groups::Persons::Age Groups::Adolescent
Medical Subject Headings::Named Groups::Persons::Age Groups::Adult
Medical Subject Headings::Named Groups::Persons::Age Groups::Child
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Check Tags::Female
Medical Subject Headings::Check Tags::Male
Medical Subject Headings::Named Groups::Persons::Age Groups::Adult::Young Adult
Medical Subject Headings::Geographicals::Geographic Locations::Europe::Spain
Medical Subject Headings::Phenomena and Processes::Immune System Phenomena::Immune System Processes::Antigen-Antibody Reactions::Cross Reactions
Medical Subject Headings::Diseases::Immune System Diseases::Hypersensitivity::Hypersensitivity, Immediate::Food Hypersensitivity
Medical Subject Headings::Technology, Industry, Agriculture::Food and Beverages::Food
Medical Subject Headings::Technology, Industry, Agriculture::Food and Beverages::Food::Fruit
Medical Subject Headings::Disciplines and Occupations::Natural Science Disciplines::Earth Sciences::Geography
Medical Subject Headings::Named Groups::Persons::Age Groups::Adult::Middle Aged
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Immunologic Techniques::Immunization
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Immunologic Techniques::Immunoassay
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Blood Proteins::Immunoproteins::Immunoglobulins::Antibodies::Immunoglobulin Isotypes::Immunoglobulin E
Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Physicochemical Phenomena::Molecular Weight
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Plant Proteins
Medical Subject Headings::Anatomy::Plant Structures::Plant Components, Aerial::Flowering Tops::Flowers::Germ Cells, Plant::Pollen
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Microchip Analytical Procedures::Microarray Analysis::Protein Array Analysis
Fecha de publicación : 7-Sep-2012
Editorial : Public Library of Science
Cita Bibliográfica: Palacín A, Rivas LA, Gómez-Casado C, Aguirre J, Tordesillas L, Bartra J, et al. The involvement of thaumatin-like proteins in plant food cross-reactivity: a multicenter study using a specific protein microarray. PLoS ONE. 2012; 7(9):e44088
Abstract: Cross-reactivity of plant foods is an important phenomenon in allergy, with geographical variations with respect to the number and prevalence of the allergens involved in this process, whose complexity requires detailed studies. We have addressed the role of thaumatin-like proteins (TLPs) in cross-reactivity between fruit and pollen allergies. A representative panel of 16 purified TLPs was printed onto an allergen microarray. The proteins selected belonged to the sources most frequently associated with peach allergy in representative regions of Spain. Sera from two groups of well characterized patients, one with allergy to Rosaceae fruit (FAG) and another against pollens but tolerant to food-plant allergens (PAG), were obtained from seven geographical areas with different environmental pollen profiles. Cross-reactivity between members of this family was demonstrated by inhibition assays. Only 6 out of 16 purified TLPs showed noticeable allergenic activity in the studied populations. Pru p 2.0201, the peach TLP (41%), chestnut TLP (24%) and plane pollen TLP (22%) proved to be allergens of probable relevance to fruit allergy, being mainly associated with pollen sensitization, and strongly linked to specific geographical areas such as Barcelona, Bilbao, the Canary Islands and Madrid. The patients exhibited >50% positive response to Pru p 2.0201 and to chestnut TLP in these specific areas. Therefore, their recognition patterns were associated with the geographical area, suggesting a role for pollen in the sensitization of these allergens. Finally, the co-sensitizations of patients considering pairs of TLP allergens were analyzed by using the co-sensitization graph associated with an allergen microarray immunoassay. Our data indicate that TLPs are significant allergens in plant food allergy and should be considered when diagnosing and treating pollen-food allergy.
Descripción : Multicenter Study; Research Support, Non-U.S. Gov't;
URI: http://hdl.handle.net/10668/1409
Versión del editor : http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0044088
DOI: 10.1371/journal.pone.0044088
ISSN : 1932-6203 (Online)
Appears in Collections:01- Artículos - IBIMA. Instituto de Investigación Biomédica de Málaga
01- Artículos - Hospital Regional de Málaga

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