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Title: Basal Sodium-Dependent Vitamin C Transporter 2 polarization in choroid plexus explant cells in normal or scorbutic conditions.
Authors: Ulloa, Viviana
Saldivia, Natalia
Ferrada, Luciano
Salazar, Katterine
Martínez, Fernando
Silva-Alvarez, Carmen
Magdalena, Rocio
Oviedo, María José
Montecinos, Hernán
Torres-Vergara, Pablo
Cifuentes, Manuel
Nualart, Francisco
metadata.dc.subject.mesh: Animals
Ascorbic Acid
Blood-Brain Barrier
Cell Membrane
Cells, Cultured
Choroid Plexus
Embryonic Development
Epithelial Cells
Gene Expression Regulation, Developmental
Glucose Transporter Type 1
Guinea Pigs
Monocarboxylic Acid Transporters
Sodium-Coupled Vitamin C Transporters
Issue Date: 8-Oct-2019
Abstract: Vitamin C is incorporated into the cerebrospinal fluid (CSF) through choroid plexus cells. While the transfer of vitamin C from the blood to the brain has been studied functionally, the vitamin C transporter, SVCT2, has not been detected in the basolateral membrane of choroid plexus cells. Furthermore, it is unknown how its expression is induced in the developing brain and modulated in scurvy conditions. We concluded that SVCT2 is intensely expressed in the second half of embryonic brain development and postnatal stages. In postnatal and adult brain, SVCT2 is highly expressed in all choroidal plexus epithelial cells, shown by colocalization with GLUT1 in the basolateral membranes and without MCT1 colocalization, which is expressed in the apical membrane. We confirmed that choroid plexus explant cells (in vitro) form a sealed epithelial structure, which polarized basolaterally, endogenous or overexpressed SVCT2. These results are reproduced in vivo by injecting hSVCT2wt-EYFP lentivirus into the CSF. Overexpressed SVCT2 incorporates AA (intraperitoneally injected) from the blood to the CSF. Finally, we observed in Guinea pig brain under scorbutic condition, that normal distribution of SVCT2 in choroid plexus may be regulated by peripheral concentrations of vitamin C. Additionally, we observed that SVCT2 polarization also depends on the metabolic stage of the choroid plexus cells.
metadata.dc.identifier.doi: 10.1038/s41598-019-50772-2
Appears in Collections:Producción 2020

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