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Title: Computational and Biological Evaluation of N-octadecyl-N'-propylsulfamide, a Selective PPARα Agonist Structurally Related to N-acylethanolamines.
Authors: Moreno-Santos, Inmaculada
Pavón, Francisco Javier
Romero-Cuevas, Miguel
Serrano, Antonia
Cano, Carolina
Suardíaz, Margarita
Decara, Juan
Suárez, Juan
Rodríguez de Fonseca, Fernando
Macías-González, Manuel
metadata.dc.contributor.authoraffiliation: [Moreno-Santos, I; Macías-González, M] Unidad de Gestión Clínica de Endocrinología y Nutrición, Hospital Universitario Virgen de la Victoria, Instituto de Investigación Biomédica de Málaga (IBIMA), Málaga, Spain. [Moreno-Santos, I; Pavón, FJ; Serrano, A; Suárez, J; Rodríguez de Fonseca, F; Macías-González, M] Centro de Investigación Biomédica en Red de Fisiopatología de la Obesidad y Nutrición (CIBERobn, CB06/03), Instituto de Salud Carlos III, Santiago de Compostela, Spain. [Pavón, FJ; Romero-Cuevas, M; Serrano, A; Suardíaz, M; Decara, J; Suárez, J; Rodríguez de Fonseca, F] Unidad de Gestión Clínica de Salud Mental, Hospital Regional Universitario de Málaga, Instituto IBIMA, Málaga, Spain. [Cano, C] Grupo Moduladores de Receptores Cannabinoides y PPARs, Instituto de Química Médica, Centro de Química Orgánica ‘‘Manuel Lora-Tamayo’’ del Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain.
Keywords: Luciferasas;Marcación de Gen;Peso Corporal;Ácidos Grasos;Genes Reporteros;Metabolismo de los Lípidos;ARN Interferente Pequeño
metadata.dc.subject.mesh: Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Physical Examination::Body Constitution::Body Weights and Measures::Body Size::Body Weight
Medical Subject Headings::Chemicals and Drugs::Lipids::Fatty Acids
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Gene Targeting
Medical Subject Headings::Phenomena and Processes::Metabolic Phenomena::Metabolism::Lipid Metabolism
Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Oxidoreductases::Luciferases
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Structures::Genome::Genome Components::Genes::Genes, Reporter
Medical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Antisense Elements (Genetics)::RNA, Antisense::RNA, Small Interfering
Issue Date: 20-Mar-2014
Publisher: Public Library of Science
Citation: Moreno-Santos I, Pavón FJ, Romero-Cuevas M, Serrano A, Cano C, Suardíaz M, et al. Computational and Biological Evaluation of N-octadecyl-N'-propylsulfamide, a Selective PPARα Agonist Structurally Related to N-acylethanolamines. PLoS ONE. 2014; 9(3):e92195
Abstract: To further understand the pharmacological properties of N-oleoylethanolamine (OEA), a naturally occurring lipid that activates peroxisome proliferator-activated receptor alpha (PPARα), we designed sulfamoyl analogs based on its structure. Among the compounds tested, N-octadecyl-N'-propylsulfamide (CC7) was selected for functional comparison with OEA. The performed studies include the following computational and biological approaches: 1) molecular docking analyses; 2) molecular biology studies with PPARα; 3) pharmacological studies on feeding behavior and visceral analgesia. For the docking studies, we compared OEA and CC7 data with crystallization data obtained with the reference PPARα agonist GW409544. OEA and CC7 interacted with the ligand-binding domain of PPARα in a similar manner to GW409544. Both compounds produced similar transcriptional activation by in vitro assays, including the GST pull-down assay and reporter gene analysis. In addition, CC7 and OEA induced the mRNA expression of CPT1a in HpeG2 cells through PPARα and the induction was avoided with PPARα-specific siRNA. In vivo studies in rats showed that OEA and CC7 had anorectic and antiobesity activity and induced both lipopenia and decreases in hepatic fat content. However, different effects were observed when measuring visceral pain; OEA produced visceral analgesia whereas CC7 showed no effects. These results suggest that OEA activity on the PPARα receptor (e.g., lipid metabolism and feeding behavior) may be dissociated from other actions at alternative targets (e.g., pain) because other non cannabimimetic ligands that interact with PPARα, such as CC7, do not reproduce the full spectrum of the pharmacological activity of OEA. These results provide new opportunities for the development of specific PPARα-activating drugs focused on sulfamide derivatives with a long alkyl chain for the treatment of metabolic dysfunction.
Description: Journal Article;
metadata.dc.identifier.doi: 10.1371/journal.pone.0092195
ISSN: 1932-6203 (Online)
Appears in Collections:01- Artículos - Hospital Regional de Málaga
01- Artículos - Hospital Virgen de la Victoria
01- Artículos - IBIMA. Instituto de Investigación Biomédica de Málaga

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