Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/1608
Título : Effects of acute versus repeated cocaine exposure on the expression of endocannabinoid signaling-related proteins in the mouse cerebellum.
Autor : Palomino, Ana
Pavón, Francisco-Javier
Blanco-Calvo, Eduardo
Serrano, Antonia
Arrabal, Sergio
Rivera, Patricia
Alén, Francisco
Vargas, Antonio
Bilbao, Ainhoa
Rubio, Leticia
Rodríguez de Fonseca, Fernando
Suárez, Juan
Filiación: [Palomino,A; Pavón,FJ; Blanco-Calvo,E; Serrano,A; Arrabal,S; Rivera,P; Vargas,A; Rodríguez de Fonseca,F; Suárez,J] Laboratorio de Investigación (Unidad de Gestión Clínica de Salud Mental), Instituto de Investigación Biomédica de Málaga, Hospital Regional Universitario de Málaga, Málaga, Spain. [Blanco-Calvo,E] Departament de Pedagogia i Psicologia, Facultat de Ciències del’Educació, Universitat de Lleida, Lleida,Spain. [Alén,F]Departamento de Psicobiología, Facultad de Psicología, Universidad Complutense, Madrid,Spain. [Bilbao,A] Institute of Psychopharmacology, Central Institute of Mental Health, Medical Faculty of Mannheim, University of Heidelberg, Mannheim, Germany. [Rubio,L] Departamento de Anatomía y Medicina Legal y Forense, Facultad de Medicina, Universidad de Málaga, Málaga,Spain.
Palabras clave : Cocaine
Sensitization
Cannabinoid
Glutamate
Tyrosine hidroxylase
Mouse
Cerebellum
Cocaina
Sensibilización del sistema nervioso central
Cannabinoides
Glutamatos
Tirosina
Ratones
Cerebelo
MeSH: Medical Subject Headings::Chemicals and Drugs::Heterocyclic Compounds::Alkaloids::Tropanes::Cocaine
Medical Subject Headings::Phenomena and Processes::Musculoskeletal and Neural Physiological Phenomena::Nervous System Physiological Phenomena::Central Nervous System Sensitization
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Amino Acids::Amino Acids, Cyclic::Amino Acids, Aromatic::Tyrosine
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Amino Acids::Amino Acids, Acidic::Glutamic Acid::Glutamates
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Amino Acids::Amino Acids, Cyclic::Amino Acids, Aromatic::Tyrosine
Medical Subject Headings::Chemicals and Drugs::Organic Chemicals::Hydrocarbons::Terpenes::Cannabinoids
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Mice
Medical Subject Headings::Anatomy::Nervous System::Central Nervous System::Brain::Rhombencephalon::Metencephalon::Cerebellum
Fecha de publicación : 5-Mar-2014
Editorial : Frontiers
Cita Bibliográfica: Palomino A, Pavón FJ, Blanco-Calvo E, Serrano A, Arrabal S, Rivera P, et al. Effects of acute versus repeated cocaine exposure on the expression of endocannabinoid signaling-related proteins in the mouse cerebellum. Front Integr Neurosci. 2014; 8:22
Abstract: Growing awareness of cerebellar involvement in addiction is based on the cerebellum's intermediary position between motor and reward, potentially acting as an interface between motivational and cognitive functions. Here, we examined the impact of acute and repeated cocaine exposure on the two main signaling systems in the mouse cerebellum: the endocannabinoid (eCB) and glutamate systems. To this end, we investigated whether eCB signaling-related gene and protein expression {cannabinoid receptor type 1 receptors and enzymes that produce [diacylglycerol lipase alpha/beta (DAGLα/β) and N-acyl phosphatidylethanolamine phospholipase D (NAPE-PLD)] and degrade [monoacylglycerol lipase (MAGL) and fatty acid amino hydrolase (FAAH)] eCB} were altered. In addition, we analyzed the gene expression of relevant components of the glutamate signaling system [glutamate synthesizing enzymes liver-type glutaminase isoform (LGA) and kidney-type glutaminase isoform (KGA), metabotropic glutamatergic receptor (mGluR3/5), NMDA-ionotropic glutamatergic receptor (NR1/2A/2B/2C) and AMPA-ionotropic receptor subunits (GluR1/2/3/4)] and the gene expression of tyrosine hydroxylase (TH), the rate-limiting enzyme in catecholamine biosynthesis, because noradrenergic terminals innervate the cerebellar cortex. Results indicated that acute cocaine exposure decreased DAGLα expression, suggesting a down-regulation of 2-arachidonylglycerol (2-AG) production, as well as gene expression of TH, KGA, mGluR3 and all ionotropic receptor subunits analyzed in the cerebellum. The acquisition of conditioned locomotion and sensitization after repeated cocaine exposure were associated with an increased NAPE-PLD/FAAH ratio, suggesting enhanced anandamide production, and a decreased DAGLβ/MAGL ratio, suggesting decreased 2-AG generation. Repeated cocaine also increased LGA gene expression but had no effect on glutamate receptors. These findings indicate that acute cocaine modulates the expression of the eCB and glutamate systems. Repeated cocaine results in normalization of glutamate receptor expression, although sustained changes in eCB is observed. We suggest that cocaine-induced alterations to cerebellar eCB should be considered when analyzing the adaptations imposed by psychostimulants that lead to addiction.
Descripción : Journal Article;
URI: http://hdl.handle.net/10668/1608
Versión del editor : http://journal.frontiersin.org/Journal/10.3389/fnint.2014.00022/abstract
DOI: 10.3389/fnint.2014.00022
ISSN : 1662-5145 (Online)
Appears in Collections:01- Artículos - IBIMA. Instituto de Investigación Biomédica de Málaga
01- Artículos - Hospital Regional de Málaga

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