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Title: Oleoylethanolamide enhances β-adrenergic-mediated thermogenesis and white-to-brown adipocyte phenotype in epididymal white adipose tissue in rat.
Authors: Suárez, Juan
Rivera, Patricia
Arrabal, Sergio
Crespillo, Ana
Serrano, Antonia
Baixeras, Elena
Pavón, Francisco J
Cifuentes, Manuel
Nogueiras, Rubén
Ballesteros, Joan
Dieguez, Carlos
Rodríguez de Fonseca, Fernando
metadata.dc.contributor.authoraffiliation: [Suárez,J; Rivera, P; Arrabal,S; Crespillo,A; Serrano,A; Baixeras,E; Pavón, FJ; Rodríguez de Fonseca,F] Laboratorio de Medicina Regenerativa, Hospital Carlos Haya-IBIMA, Málaga, Spain. [Suárez,J; Nogueiras,R; Dieguez,C; Rodríguez de Fonseca,F] Centro de Investigación Biomédica en Red Fisiopatología de la Obesidad y Nutrición (CIBERobn), Instituto de Salud Carlos III, Madrid, Spain. [Cifuentes,M] Departamento de Biología Celular, Genética y Fisiología, Universidad de Málaga, Málaga, Spain. Centro de Investigaciones Biomédicas en Red de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Instituto de Salud Carlos III, Madrid, Spain. [Nogueiras,R] Department of Physiology, School of Medicine-CIMUS, University of Santiago de Compostela-Instituto de Investigación Sanitaria, Santiago de Compostela, Spain. [Ballesteros,J] ViviaBiotech S.L., Campanillas, Spain.
Keywords: Peroxisome proliferator-activated receptor alpha;β3-adrenergic receptor;Thermogenesis;β-oxidation;Adipocyte;PPAR alfa;Termogénesis;Ratas;Regulación del apetito;Peso corporal
metadata.dc.subject.mesh: Medical Subject Headings::Anatomy::Cells::Connective Tissue Cells::Adipocytes
Medical Subject Headings::Phenomena and Processes::Physiological Phenomena::Physiological Processes::Body Temperature Regulation::Thermogenesis
Medical Subject Headings::Anatomy::Cells::Connective Tissue Cells::Adipocytes
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Rats
Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Oxidoreductases::Oxygenases::Mixed Function Oxygenases::Fatty Acid Desaturases::Stearoyl-CoA Desaturase
Medical Subject Headings::Phenomena and Processes::Physiological Phenomena::Nutritional Physiological Phenomena::Hunger::Appetite::Appetite Regulation
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Anthropometry::Body Weights and Measures::Body Size::Body Weight
Issue Date: 7-Jan-2014
Publisher: Company of Biologists
Citation: Suárez J, Rivera P, Arrabal S, Crespillo A, Serrano A, Baixeras E, et al. Oleoylethanolamide enhances β-adrenergic-mediated thermogenesis and white-to-brown adipocyte phenotype in epididymal white adipose tissue in rat. Dis Model Mech. 2014; 7(1):129-41
Abstract: β-adrenergic receptor activation promotes brown adipose tissue (BAT) β-oxidation and thermogenesis by burning fatty acids during uncoupling respiration. Oleoylethanolamide (OEA) can inhibit feeding and stimulate lipolysis by activating peroxisome proliferator-activating receptor-α (PPARα) in white adipose tissue (WAT). Here we explore whether PPARα activation potentiates the effect of β3-adrenergic stimulation on energy balance mediated by the respective agonists OEA and CL316243. The effect of this pharmacological association on feeding, thermogenesis, β-oxidation, and lipid and cholesterol metabolism in epididymal (e)WAT was monitored. CL316243 (1 mg/kg) and OEA (5 mg/kg) co-administration over 6 days enhanced the reduction of both food intake and body weight gain, increased the energy expenditure and reduced the respiratory quotient (VCO2/VO2). This negative energy balance agreed with decreased fat mass and increased BAT weight and temperature, as well as with lowered plasma levels of triglycerides, cholesterol, nonessential fatty acids (NEFAs), and the adipokines leptin and TNF-α. Regarding eWAT, CL316243 and OEA treatment elevated levels of the thermogenic factors PPARα and UCP1, reduced p38-MAPK phosphorylation, and promoted brown-like features in the white adipocytes: the mitochondrial (Cox4i1, Cox4i2) and BAT (Fgf21, Prdm16) genes were overexpressed in eWAT. The enhancement of the fatty-acid β-oxidation factors Cpt1b and Acox1 in eWAT was accompanied by an upregulation of de novo lipogenesis and reduced expression of the unsaturated-fatty-acid-synthesis enzyme gene, Scd1. We propose that the combination of β-adrenergic and PPARα receptor agonists promotes therapeutic adipocyte remodelling in eWAT, and therefore has a potential clinical utility in the treatment of obesity.
Description: Journal Article; Research Support, Non-U.S. Gov't;
metadata.dc.identifier.doi: 10.1242/dmm.013110
ISSN: 1754-8411 (Online)
1754-8403 (Print)
Appears in Collections:01- Artículos - Hospital Regional de Málaga

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