Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/1639
Título : Selection of Reference Genes for Quantitative Real Time PCR (qPCR) Assays in Tissue from Human Ascending Aorta.
Autor : Rueda-Martínez, Carmen
Lamas, Óscar
Mataró, María José
Robledo-Carmona, Juan
Sánchez-Espín, Gemma
Jiménez-Navarro, Manuel
Such-Martínez, Miguel
Fernández, Borja
Filiación: [Rueda-Martínez,C; Lamas,O; Mataró,MJ; Robledo-Carmona,J; Sánchez-Espín,G; Jiménez-Navarro,M; Such-Martínez,M] UGC del Corazón, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Clínico Universitario Virgen de la Victoria, Málaga, Spain. [Jiménez-Navarro,M] Departamento de Medicina, Facultad de Medicina, Universidad de Málaga, Spain. [Fernández, B] Departamento de Biología Animal, Instituto de Investigación Biomédica de Málaga (IBIMA), Facultad de Ciencias, Universidad de Málaga, Spain.
Palabras clave : Enfermedades de las Válvulas Cardíacas
Válvula Aórtica
Algoritmos
Aneurisma de la Aorta
Reacción en Cadena en Tiempo Real de la Polimerasa
Demografía
Expresión Génica
Dilatación Patológica
Reacción en Cadena de la Polimerasa
ADN Complementario
Desnaturalización de Ácido Nucleico
Humanos
MeSH: Medical Subject Headings::Diseases::Cardiovascular Diseases::Heart Diseases::Heart Valve Diseases
Medical Subject Headings::Anatomy::Cardiovascular System::Heart::Heart Valves::Aortic Valve
Medical Subject Headings::Information Science::Information Science::Computing Methodologies::Algorithms
Medical Subject Headings::Anatomy::Cardiovascular System::Blood Vessels::Arteries::Aorta
Medical Subject Headings::Diseases::Cardiovascular Diseases::Vascular Diseases::Aortic Diseases::Aortic Aneurysm
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Nucleic Acid Amplification Techniques::Polymerase Chain Reaction::Real-Time Polymerase Chain Reaction
Medical Subject Headings::Health Care::Population Characteristics::Demography
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Gene Expression
Medical Subject Headings::Diseases::Pathological Conditions, Signs and Symptoms::Pathological Conditions, Anatomical::Dilatation, Pathologic
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Nucleic Acid Amplification Techniques::Polymerase Chain Reaction
Medical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::DNA::DNA, Single-Stranded::DNA, Complementary
Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Biochemical Processes::Nucleic Acid Denaturation
Medical Subject Headings::Anatomy::Tissues
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Fecha de publicación : 19-May-2014
Editorial : Public Library of Science
Cita Bibliográfica: Rueda-Martínez C, Lamas O, Mataró MJ, Robledo-Carmona J, Sánchez-Espín G, Jiménez-Navarro M, et al. Selection of Reference Genes for Quantitative Real Time PCR (qPCR) Assays in Tissue from Human Ascending Aorta. PLoS ONE. 2014; 9(5):e97449
Abstract: Dilatation of the ascending aorta (AAD) is a prevalent aortopathy that occurs frequently associated with bicuspid aortic valve (BAV), the most common human congenital cardiac malformation. The molecular mechanisms leading to AAD associated with BAV are still poorly understood. The search for differentially expressed genes in diseased tissue by quantitative real-time PCR (qPCR) is an invaluable tool to fill this gap. However, studies dedicated to identify reference genes necessary for normalization of mRNA expression in aortic tissue are scarce. In this report, we evaluate the qPCR expression of six candidate reference genes in tissue from the ascending aorta of 52 patients with a variety of clinical and demographic characteristics, normal and dilated aortas, and different morphologies of the aortic valve (normal aorta and normal valve n = 30; dilated aorta and normal valve n = 10; normal aorta and BAV n = 4; dilated aorta and BAV n = 8). The expression stability of the candidate reference genes was determined with three statistical algorithms, GeNorm, NormFinder and Bestkeeper. The expression analyses showed that the most stable genes for the three algorithms employed were CDKN1β, POLR2A and CASC3, independently of the structure of the aorta and the valve morphology. In conclusion, we propose the use of these three genes as reference genes for mRNA expression analysis in human ascending aorta. However, we suggest searching for specific reference genes when conducting qPCR experiments with new cohort of samples.
Descripción : Journal Article;
URI: http://hdl.handle.net/10668/1639
Versión del editor : http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0097449;jsessionid=E2EE22879D14499FF2D7BEC41768591E
DOI: 10.1371/journal.pone.0097449
ISSN : 1932-6203 (Online)
Appears in Collections:01- Artículos - IBIMA. Instituto de Investigación Biomédica de Málaga
01- Artículos - Hospital Virgen de la Victoria

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