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dc.contributor.authorRueda-Martínez, Carmen-
dc.contributor.authorLamas, Óscar-
dc.contributor.authorMataró, María José-
dc.contributor.authorRobledo-Carmona, Juan-
dc.contributor.authorSánchez-Espín, Gemma-
dc.contributor.authorJiménez-Navarro, Manuel-
dc.contributor.authorSuch-Martínez, Miguel-
dc.contributor.authorFernández, Borja-
dc.identifier.citationRueda-Martínez C, Lamas O, Mataró MJ, Robledo-Carmona J, Sánchez-Espín G, Jiménez-Navarro M, et al. Selection of Reference Genes for Quantitative Real Time PCR (qPCR) Assays in Tissue from Human Ascending Aorta. PLoS ONE. 2014; 9(5):e97449es
dc.identifier.issn1932-6203 (Online)-
dc.descriptionJournal Article;es
dc.description.abstractDilatation of the ascending aorta (AAD) is a prevalent aortopathy that occurs frequently associated with bicuspid aortic valve (BAV), the most common human congenital cardiac malformation. The molecular mechanisms leading to AAD associated with BAV are still poorly understood. The search for differentially expressed genes in diseased tissue by quantitative real-time PCR (qPCR) is an invaluable tool to fill this gap. However, studies dedicated to identify reference genes necessary for normalization of mRNA expression in aortic tissue are scarce. In this report, we evaluate the qPCR expression of six candidate reference genes in tissue from the ascending aorta of 52 patients with a variety of clinical and demographic characteristics, normal and dilated aortas, and different morphologies of the aortic valve (normal aorta and normal valve n = 30; dilated aorta and normal valve n = 10; normal aorta and BAV n = 4; dilated aorta and BAV n = 8). The expression stability of the candidate reference genes was determined with three statistical algorithms, GeNorm, NormFinder and Bestkeeper. The expression analyses showed that the most stable genes for the three algorithms employed were CDKN1β, POLR2A and CASC3, independently of the structure of the aorta and the valve morphology. In conclusion, we propose the use of these three genes as reference genes for mRNA expression analysis in human ascending aorta. However, we suggest searching for specific reference genes when conducting qPCR experiments with new cohort of
dc.description.sponsorshipThis work was supported in part by grants PI-0689/2010 (Consejería de Salud, Junta de Andalucía, Spain), P10-CTS-06068 (Consejería de Innovación, Ciencia y Empresa, Junta de Andalucía, Spain), and Red de Investigación Cardiovascular (RIC) from Redes Temáticas de Investigación Cooperativa (RETIC)es
dc.publisherPublic Library of Sciencees
dc.relation.ispartofPloS Onees
dc.subjectEnfermedades de las Válvulas Cardíacases
dc.subjectVálvula Aórticaes
dc.subjectAneurisma de la Aortaes
dc.subjectReacción en Cadena en Tiempo Real de la Polimerasaes
dc.subjectExpresión Génicaes
dc.subjectDilatación Patológicaes
dc.subjectReacción en Cadena de la Polimerasaes
dc.subjectADN Complementarioes
dc.subjectDesnaturalización de Ácido Nucleicoes
dc.subject.meshMedical Subject Headings::Diseases::Cardiovascular Diseases::Heart Diseases::Heart Valve Diseaseses
dc.subject.meshMedical Subject Headings::Anatomy::Cardiovascular System::Heart::Heart Valves::Aortic Valvees
dc.subject.meshMedical Subject Headings::Information Science::Information Science::Computing Methodologies::Algorithmses
dc.subject.meshMedical Subject Headings::Anatomy::Cardiovascular System::Blood Vessels::Arteries::Aortaes
dc.subject.meshMedical Subject Headings::Diseases::Cardiovascular Diseases::Vascular Diseases::Aortic Diseases::Aortic Aneurysmes
dc.subject.meshMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Nucleic Acid Amplification Techniques::Polymerase Chain Reaction::Real-Time Polymerase Chain Reactiones
dc.subject.meshMedical Subject Headings::Health Care::Population Characteristics::Demographyes
dc.subject.meshMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Gene Expressiones
dc.subject.meshMedical Subject Headings::Diseases::Pathological Conditions, Signs and Symptoms::Pathological Conditions, Anatomical::Dilatation, Pathologices
dc.subject.meshMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Nucleic Acid Amplification Techniques::Polymerase Chain Reactiones
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::DNA::DNA, Single-Stranded::DNA, Complementaryes
dc.subject.meshMedical Subject Headings::Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Biochemical Processes::Nucleic Acid Denaturationes
dc.subject.meshMedical Subject Headings::Anatomy::Tissueses
dc.subject.meshMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humanses
dc.titleSelection of Reference Genes for Quantitative Real Time PCR (qPCR) Assays in Tissue from Human Ascending
dc.rights.accessRightsAcceso abiertoes
dc.contributor.authoraffiliation[Rueda-Martínez,C; Lamas,O; Mataró,MJ; Robledo-Carmona,J; Sánchez-Espín,G; Jiménez-Navarro,M; Such-Martínez,M] UGC del Corazón, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Clínico Universitario Virgen de la Victoria, Málaga, Spain. [Jiménez-Navarro,M] Departamento de Medicina, Facultad de Medicina, Universidad de Málaga, Spain. [Fernández, B] Departamento de Biología Animal, Instituto de Investigación Biomédica de Málaga (IBIMA), Facultad de Ciencias, Universidad de Málaga,
Appears in Collections:01- Artículos - IBIMA. Instituto de Investigación Biomédica de Málaga
01- Artículos - Hospital Virgen de la Victoria

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