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http://hdl.handle.net/10668/1703| Title: | Acute oxygen sensing in heme oxygenase-2 null mice. |
| Authors: | Ortega-Sáenz, Patricia Pascual, Alberto Gómez-Díaz, Raquel López-Barneo, José |
| metadata.dc.contributor.authoraffiliation: | Laboratorio de Investigaciones Biomédicas, Hospital Universitario Virgen del Rocío, Universidad de Sevilla, Sevilla,Spain |
| Keywords: | Animales;Animales Recién Nacidos;Calcio;Cuerpo Carotídeo;Catecolaminas;Células Cromafines;Ciclofilinas;Expresión Génica;Hemo Oxigenasa (Deciclizadora);Hemo-Oxigenasa 1;Subunidades alfa de los Canales de Potasio de Gran Conductancia Activados por Calcio;Canales de Potasio de Gran Conductancia Activados por el Calcio;Ratones;Ratones Consanguíneos C57BL;Ratones Noqueados;Tamaño de los Órganos;Oxígeno;Presión Parcial;Técnicas de Placa-Clamp;Péptidos;Bloqueadores de los Canales de Potasio;Ratas;Médula Suprarrenal |
| metadata.dc.subject.mesh: | Medical Subject Headings::Organisms::Eukaryota::Animals Medical Subject Headings::Organisms::Eukaryota::Animals::Animal Population Groups::Animals, Newborn Medical Subject Headings::Diseases::Pathological Conditions, Signs and Symptoms::Signs and Symptoms::Signs and Symptoms, Respiratory::Anoxia Medical Subject Headings::Chemicals and Drugs::Inorganic Chemicals::Elements::Metals, Alkaline Earth::Calcium Medical Subject Headings::Anatomy::Nervous System::Neurons::Neurons, Afferent::Sensory Receptor Cells::Chemoreceptor Cells::Paraganglia, Nonchromaffin::Carotid Body Medical Subject Headings::Chemicals and Drugs::Organic Chemicals::Amines::Catecholamines Medical Subject Headings::Anatomy::Cells::Chromaffin Cells Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Isomerases::cis-trans-Isomerases::Peptidylprolyl Isomerase::Immunophilins::Cyclophilins Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Gene Expression Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Oxidoreductases::Oxygenases::Mixed Function Oxygenases::Heme Oxygenase (Decyclizing) Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Oxidoreductases::Oxygenases::Mixed Function Oxygenases::Heme Oxygenase (Decyclizing)::Heme Oxygenase-1 Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Carrier Proteins::Membrane Transport Proteins::Ion Channels::Potassium Channels::Potassium Channels, Calcium-Activated::Large-Conductance Calcium-Activated Potassium Channels Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Mice Medical Subject Headings::Organisms::Eukaryota::Animals::Animal Population Groups::Animals, Inbred Strains::Mice, Inbred Strains::Mice, Inbred C57BL Medical Subject Headings::Organisms::Eukaryota::Animals::Animal Population Groups::Animals, Genetically Modified::Mice, Transgenic::Mice, Knockout Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Physical Examination::Body Constitution::Body Weights and Measures::Organ Size Medical Subject Headings::Chemicals and Drugs::Inorganic Chemicals::Elements::Chalcogens::Oxygen Medical Subject Headings::Phenomena and Processes::Physical Phenomena::Mechanical Phenomena::Pressure::Partial Pressure Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Cytological Techniques::Patch-Clamp Techniques Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Peptides Medical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Molecular Mechanisms of Pharmacological Action::Membrane Transport Modulators::Potassium Channel Blockers Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Rats Tyrosine 3-Monooxygenase Medical Subject Headings::Anatomy::Endocrine System::Endocrine Glands::Adrenal Glands::Adrenal Medulla |
| Issue Date: | Oct-2006 |
| Publisher: | Rockefeller University Press |
| Citation: | Ortega-Sáenz P, Pascual A, Gómez-Díaz R, López-Barneo J. Acute oxygen sensing in heme oxygenase-2 null mice. J Gen Physiol. 2006; 128(4):405-1 |
| Abstract: | Hemeoxygenase-2 (HO-2) is an antioxidant enzyme that can modulate recombinant maxi-K(+) channels and has been proposed to be the acute O(2) sensor in the carotid body (CB). We have tested the physiological contribution of this enzyme to O(2) sensing using HO-2 null mice. HO-2 deficiency leads to a CB phenotype characterized by organ growth and alteration in the expression of stress-dependent genes, including the maxi-K(+) channel alpha-subunit. However, sensitivity to hypoxia of CB is remarkably similar in HO-2 null animals and their control littermates. Moreover, the response to hypoxia in mouse and rat CB cells was maintained after blockade of maxi-K(+) channels with iberiotoxin. Hypoxia responsiveness of the adrenal medulla (AM) (another acutely responding O(2)-sensitive organ) was also unaltered by HO-2 deficiency. Our data suggest that redox disregulation resulting from HO-2 deficiency affects maxi-K(+) channel gene expression but it does not alter the intrinsic O(2) sensitivity of CB or AM cells. Therefore, HO-2 is not a universally used acute O(2) sensor. |
| Description: | Journal Article; Research Support, Non-U.S. Gov't;. A Pascual is an investigator of the “Ramón y Cajal” program. J. López-Barneo received the “Ayuda a la Investigación 2000” of the Juan March Foundation |
| URI: | http://hdl.handle.net/10668/1703 |
| metadata.dc.relation.publisherversion: | http://jgp.rupress.org/content/128/4/405.long#abstract-1 |
| metadata.dc.identifier.doi: | 10.1085/jgp.200609591 |
| ISSN: | 1540-7748 (Online) 0022-1295 (Print) |
| Appears in Collections: | 01- Artículos - Hospital Virgen del Rocío |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| OrtegaSaenz_AcuteOxygen.pdf | 998,11 kB | Adobe PDF | View/Open |
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