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Title: Collagen analogs with phosphorylcholine are inflammation-suppressing scaffolds for corneal regeneration from alkali burns in mini-pigs.
Authors: Simpson, Fiona C
McTiernan, Christopher D
Islam, Mohammad Mirazul
Buznyk, Oleksiy
Lewis, Philip N
Meek, Keith M
Haagdorens, Michel
Audiger, Cindy
Lesage, Sylvie
Gueriot, François-Xavier
Brunette, Isabelle
Robert, Marie-Claude
Olsen, David
Koivusalo, Laura
Liszka, Aneta
Fagerholm, Per
Gonzalez-Andrades, Miguel
Griffith, May
metadata.dc.subject.mesh: Alkalies
Biocompatible Materials
Burns, Chemical
Mice, Inbred C57BL
Swine, Miniature
Issue Date: 21-May-2021
Abstract: The long-term survival of biomaterial implants is often hampered by surgery-induced inflammation that can lead to graft failure. Considering that most corneas receiving grafts are either pathological or inflamed before implantation, the risk of rejection is heightened. Here, we show that bioengineered, fully synthetic, and robust corneal implants can be manufactured from a collagen analog (collagen-like peptide-polyethylene glycol hybrid, CLP-PEG) and inflammation-suppressing polymeric 2-methacryloyloxyethyl phosphorylcholine (MPC) when stabilized with the triazine-based crosslinker 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride. The resulting CLP-PEG-MPC implants led to reduced corneal swelling, haze, and neovascularization in comparison to CLP-PEG only implants when grafted into a mini-pig cornea alkali burn model of inflammation over 12 months. Implants incorporating MPC allowed for faster nerve regeneration and recovery of corneal sensation. CLP-PEG-MPC implants appear to be at a more advanced stage of regeneration than the CLP-PEG only implants, as evidenced by the presence of higher amounts of cornea-specific type V collagen, and a corresponding decrease in the presence of extracellular vesicles and exosomes in the corneal stroma, in keeping with the amounts present in healthy, unoperated corneas.
metadata.dc.identifier.doi: 10.1038/s42003-021-02108-y
Appears in Collections:Producción 2020

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