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Title: PARP inhibition attenuates histopathological lesion in ischemia/reperfusion renal mouse model after cold prolonged ischemia.
Authors: Moral, Raimundo M G del
Gómez-Morales, Mercedes
Hernández-Cortés, Pedro
Aguilar, David
Caballero, Trinidad
Aneiros-Fernández, Jose
Caba-Molina, Mercedes
Rodríguez-Martínez, M Dolores
Peralta, Andreina
Galindo-Moreno, Pablo
Osuna, Antonio
Oliver, F Javier
Moral, Raimundo G del
O'Valle, Francisco
metadata.dc.contributor.authoraffiliation: [Moral,RMG del; Gómez-Morales,M; Aneiros-Fernández,J; Caba-Molina,M; Moral, RG del] Provincial UGC Intercentre, Department of ICU, Granada, Spain. [Hernández-Cortés,P] Department of Traumatology and Orthopedic Surgery, IBIMER, “San Cecilio” Clinical Hospital and University of Granada, Spain. [Aguilar,D; Caballero,T; Rodríguez-Martínez,MD; O’Valle,F] Department of Pathology and IBIMER, School of Medicine, University of Granada, Spain. [Peralta,A; Oliver,FJ] Institute of Parasitology and Biomedicine, CSIC, Granada, Spain. [Galindo-Moreno,P] Oral Surgery and Implant Dentistry Department, School of Dentistry, University of Granada, Spain. [Galindo-Moreno,P] Department of Periodontics and Oral Medicine School of Dentistry, University of Michigan, Ann Arbor, MI, USA. [Osuna,A] Department of Nephrology, “Virgen de las Nieves” Universitary Hospital, Granada, Spain
Keywords: poly(ADP-ribose)polymerase-1, mouse;3,4-dihydro-5-(4-(1-piperidinyl)butoxy)-1(2H)-isoquinolinone;Frio;Isquemia fría;Riñón;Necrosis tubular aguda;Ratones;Ratones consanguíneos C57BL;Ratones noqueados;Microscopía electrónica;Piperidinas;Poli(ADP-Ribosa) polimerasas;Daño por reperfusión;Animales
metadata.dc.subject.mesh: Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Electrophoresis::Blotting, Western
Medical Subject Headings::Phenomena and Processes::Physical Phenomena::Thermodynamics::Temperature::Cold Temperature
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Preservation, Biological::Tissue Preservation::Cold Ischemia
Medical Subject Headings::Chemicals and Drugs::Heterocyclic Compounds::Heterocyclic Compounds, 2-Ring::Isoquinolines
Medical Subject Headings::Anatomy::Urogenital System::Urinary Tract::Kidney
Medical Subject Headings::Diseases::Female Urogenital Diseases and Pregnancy Complications::Female Urogenital Diseases::Urologic Diseases::Kidney Diseases::Renal Insufficiency::Acute Kidney Injury::Kidney Tubular Necrosis, Acute
Medical Subject Headings::Check Tags::Male
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Mice
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Mice::Mice, Inbred Strains::Mice, Inbred C57BL
Medical Subject Headings::Organisms::Eukaryota::Animals::Animal Population Groups::Animals, Genetically Modified::Mice, Transgenic::Mice, Knockout
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Microscopy::Microscopy, Electron
Medical Subject Headings::Chemicals and Drugs::Heterocyclic Compounds::Heterocyclic Compounds, 1-Ring::Piperidines
Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Transferases::Glycosyltransferases::Pentosyltransferases::ADP Ribose Transferases::Poly(ADP-ribose) Polymerases
Medical Subject Headings::Diseases::Pathological Conditions, Signs and Symptoms::Pathologic Processes::Postoperative Complications::Reperfusion Injury
Medical Subject Headings::Organisms::Eukaryota::Animals
Issue Date: 2013
Publisher: Hindawi Publishing Corporation
Citation: Moral RMG del, Gómez-Morales M, Hernández-Cortés P, Aguilar D, Caballero T, Aneiros-Fernández J, et al. PARP inhibition attenuates histopathological lesion in ischemia/reperfusion renal mouse model after cold prolonged ischemia. ScientificWorldJournal; 2013:486574
Abstract: We test the hypothesis that PARP inhibition can decrease acute tubular necrosis (ATN) and other renal lesions related to prolonged cold ischemia/reperfusion (IR) in kidneys preserved at 4°C in University of Wisconsin (UW) solution. Material and Methods. We used 30 male Parp1(+/+) wild-type and 15 male Parp1(0/0) knockout C57BL/6 mice. Fifteen of these wild-type mice were pretreated with 3,4-dihydro-5-[4-(1-piperidinyl)butoxyl]-1(2H)-isoquinolinone (DPQ) at a concentration of 15 mg/kg body weight, used as PARP inhibitor. Subgroups of mice were established (A: IR 45 min/6 h; B: IR + 48 h in UW solution; and C: IR + 48 h in UW solution plus DPQ). We processed samples for morphological, immunohistochemical, ultrastructural, and western-blotting studies. Results. Prolonged cold ischemia time in UW solution increased PARP-1 expression and kidney injury. Preconditioning with PARP inhibitor DPQ plus DPQ supplementation in UW solution decreased PARP-1 nuclear expression in renal tubules and renal damage. Parp1(0/0) knockout mice were more resistant to IR-induced renal lesion. In conclusion, PARP inhibition attenuates ATN and other IR-related renal lesions in mouse kidneys under prolonged cold storage in UW solution. If confirmed, these data suggest that pharmacological manipulation of PARP activity may have salutary effects in cold-stored organs at transplantation.
Description: Journal Article; Research Support, Non-U.S. Gov't;
metadata.dc.identifier.doi: 10.1155/2013/486574
ISSN: 1537-744X (Online)
Appears in Collections:01- Artículos - Hospital San Cecilio
01- Artículos - Hospital Virgen de las Nieves

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