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Title: Characterization of microevolution events in Mycobacterium tuberculosis strains involved in recent transmission clusters.
Authors: Pérez-Lago, Laura
Herranz, Marta
Martínez-Lirola, Miguel
Bouza, Emilio
García de Viedma, Darío
metadata.dc.contributor.authoraffiliation: [Pérez-Lago,L; Herranz,M; Bouza,E; García de Viedma,D] Servicio de Microbiología y Enfermedades Infecciosas, Hospital Gregorio Marañón, Madrid, Spain. [Pérez-Lago,L; Herranz,M; Bouza,E; García de Viedma,D] Instituto de Investigación Biomédica Gregorio Marañon, Madrid, Spain. [Herranz,M; Bouza,E; García de Viedma,D] CIBER Enfermedades Respiratorias- CIBERES. [Martínez-Lirola,M] Complejo Hospitalario Torrecardenas, Almería, Spain. INDAL-TB Group
Keywords: Mycobacterium tuberculosis;Molecular Typing;Evolution, Molecular;DNA Transposable Elements;Tuberculosis;Mycobacterium tuberculosis;Tipificación molecular;Evolución molecular;Elementos transponibles de ADN
metadata.dc.subject.mesh: Medical Subject Headings::Phenomena and Processes::Biological Phenomena::Biological Processes::Biological Evolution::Evolution, Molecular
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Molecular Typing
Medical Subject Headings::Organisms::Bacteria::Gram-Positive Bacteria::Gram-Positive Rods::Gram-Positive Asporogenous Rods::Gram-Positive Asporogenous Rods, Regular::Mycobacteriaceae::Mycobacterium::Mycobacterium tuberculosis
Medical Subject Headings::Diseases::Bacterial Infections and Mycoses::Bacterial Infections::Gram-Positive Bacterial Infections::Actinomycetales Infections::Mycobacterium Infections::Tuberculosis
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Structures::Genome::Genome Components::Interspersed Repetitive Sequences::DNA Transposable Elements
Issue Date: 21-Nov-2011
Publisher: American Society for Microbiology
Citation: Pérez-Lago L, Herranz M, Lirola MM, Bouza E, García de Viedma D. Characterization of microevolution events in Mycobacterium tuberculosis strains involved in recent transmission clusters. J. Clin. Microbiol.. 2011 ; 49(11):3771-6
Abstract: Under certain circumstances, it is possible to identify clonal variants of Mycobacterium tuberculosis infecting a single patient, probably as a result of subtle genetic rearrangements in part of the bacillary population. We systematically searched for these microevolution events in a different context, namely, recent transmission chains. We studied the clustered cases identified using a population-based universal molecular epidemiology strategy over a 5-year period. Clonal variants of the reference strain defining the cluster were found in 9 (12%) of the 74 clusters identified after the genotyping of 612 M. tuberculosis isolates by IS6110 restriction fragment length polymorphism analysis and mycobacterial interspersed repetitive units-variable-number tandem repeat typing. Clusters with microevolution events were epidemiologically supported and involved 4 to 9 cases diagnosed over a 1- to 5-year period. The IS6110 insertion sites from 16 representative isolates of reference and microevolved variants were mapped by ligation-mediated PCR in order to characterize the genetic background involved in microevolution. Both intragenic and intergenic IS6110 locations resulted from these microevolution events. Among those cases of IS6110 locations in intergenic regions which could have an effect on the regulation of adjacent genes, we identified the overexpression of cytochrome P450 in one microevolved variant using quantitative real-time reverse transcription-PCR. Our results help to define the frequency with which microevolution can be expected in M. tuberculosis transmission chains. They provide a snapshot of the genetic background of these subtle rearrangements and identify an event in which IS6110-mediated microevolution in an isogenic background has functional consequences.
Description: Journal Article
metadata.dc.identifier.doi: 10.1128/JCM.01285-11
ISSN: 1098-660X (Online)
0095-1137 (Print)
Appears in Collections:01- Artículos - Complejo Hospitalario Torrecárdenas

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