Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/1901
Título : Spanish multicenter study of the epidemiology and mechanisms of amoxicillin-clavulanate resistance in Escherichia coli.
Autor : Ortega, Adriana
Oteo, Jesús
Aranzamendi-Zaldumbide, Maitane
Bartolomé, Rosa M
Bou, Germán
Cercenado, Emilia
Conejo, M Carmen
González-López, Juan José
Marín, Mercedes
Martínez-Martínez, Luis
Merino, María
Navarro, Ferran
Oliver, Antonio
Pascual, Alvaro
Rivera, Alba
Rodríguez-Baño, Jesús
Weber, Irene
Aracil, Belén
Campos, José
Filiación: [Ortega,A; Oteo,J; Aracil,B; Campos,J] Laboratorio de Antibióticos, Bacteriología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain. [Aranzamendi-Zaldumbide,M; Martínez-Martínez,L] Servicio de Microbiología, Hospital Universitario Marqués de Valdecilla-IFIMAV, Santander, Spain. [Bartolomé,RM; González-López,JJ] Servei de Microbiologia, Hospital Vall d'Hebrón, Universitat Autònoma de Barcelona, Barcelona, Spain. [Bou,G; Merino,M] Servicio de Microbiología-INIBIC, Complejo Hospitalario Universitario A Coruña, Galicia, Spain. [Cercenado,E; Marín,M] Servicio de Microbiología, Hospital General Universitario Gregorio Marañón, Madrid, Spain. [Conejo,MC; Pascual,A] Departamento de Microbiología, Universidad de Sevilla, Seville, Spain. [Martínez-Martínez,L] Departamento de Biología Molecular, Universidad de Cantabria, Santander, Spain. [Navarro,F; Rivera,A] Servei de Microbiologia, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain. [Navarro,F] Departament de Genètica i de Microbiología, Universitat Autònoma de Barcelona, Institut d′Investigacions Biomèdiques Sant Pau, Barcelona, Spain. [Oliver,A; Weber,I] Servicio de Microbiología, Hospital Son Espases, Palma de Mallorca, Spain. [Pascual,A; Rodríguez-Baño,J] Unidad Clínica de Enfermedades Infecciosas y Microbiología, Hospital Universitario Virgen Macarena, Seville, Spain. [Rodríguez-Baño,J] Departamento de Medicina, Universidad de Sevilla, Seville, Spain. [Campos,J] Consejo Superior de Investigaciones Científicas, Madrid, Spain.
Palabras clave : Combinación de amoxicilina clavulanato potásico
Farmacorresistencia bacteriana
Infecciones por Escherichia coli
Epidemiología molecular
Estudios prospectivos
España
MeSH: Medical Subject Headings::Phenomena and Processes::Microbiological Phenomena::Bacterial Physiological Phenomena::Drug Resistance, Bacterial
Medical Subject Headings::Organisms::Bacteria::Gram-Negative Bacteria::Gram-Negative Facultatively Anaerobic Rods::Enterobacteriaceae::Escherichia::Escherichia coli
Medical Subject Headings::Diseases::Bacterial Infections and Mycoses::Bacterial Infections::Gram-Negative Bacterial Infections::Enterobacteriaceae Infections::Escherichia coli Infections
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Molecular Epidemiology
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Epidemiologic Study Characteristics as Topic::Epidemiologic Studies::Cohort Studies::Longitudinal Studies::Prospective Studies
Medical Subject Headings::Geographicals::Geographic Locations::Europe::Spain
Medical Subject Headings::Chemicals and Drugs::Organic Chemicals::Amides::Lactams::beta-Lactams::Clavulanic Acids::Clavulanic Acid::Amoxicillin-Potassium Clavulanate Combination
Fecha de publicación : Jul-2012
Editorial : American Society for Microbiology
Cita Bibliográfica: Ortega A, Oteo J, Aranzamendi-Zaldumbide M, Bartolomé RM, Bou G, Cercenado E, et al. Spanish multicenter study of the epidemiology and mechanisms of amoxicillin-clavulanate resistance in Escherichia coli. Antimicrob. Agents Chemother.. 2012 ; 56(7):3576-81
Abstract: We conducted a prospective multicenter study in Spain to characterize the mechanisms of resistance to amoxicillin-clavulanate (AMC) in Escherichia coli. Up to 44 AMC-resistant E. coli isolates (MIC ≥ 32/16 μg/ml) were collected at each of the seven participant hospitals. Resistance mechanisms were characterized by PCR and sequencing. Molecular epidemiology was studied by pulsed-field gel electrophoresis (PFGE) and by multilocus sequence typing. Overall AMC resistance was 9.3%. The resistance mechanisms detected in the 257 AMC-resistant isolates were OXA-1 production (26.1%), hyperproduction of penicillinase (22.6%), production of plasmidic AmpC (19.5%), hyperproduction of chromosomic AmpC (18.3%), and production of inhibitor-resistant TEM (IRT) (17.5%). The IRTs identified were TEM-40 (33.3%), TEM-30 (28.9%), TEM-33 (11.1%), TEM-32 (4.4%), TEM-34 (4.4%), TEM-35 (2.2%), TEM-54 (2.2%), TEM-76 (2.2%), TEM-79 (2.2%), and the new TEM-185 (8.8%). By PFGE, a high degree of genetic diversity was observed although two well-defined clusters were detected in the OXA-1-producing isolates: the C1 cluster consisting of 19 phylogroup A/sequence type 88 [ST88] isolates and the C2 cluster consisting of 19 phylogroup B2/ST131 isolates (16 of them producing CTX-M-15). Each of the clusters was detected in six different hospitals. In total, 21.8% of the isolates were serotype O25b/phylogroup B2 (O25b/B2). AMC resistance in E. coli is widespread in Spain at the hospital and community levels. A high prevalence of OXA-1 was found. Although resistant isolates were genetically diverse, clonality was linked to OXA-1-producing isolates of the STs 88 and 131. Dissemination of IRTs was frequent, and the epidemic O25b/B2/ST131 clone carried many different mechanisms of AMC resistance.
Descripción : Journal Article; Multicenter Study; Research Support, Non-U.S. Gov't;
URI: http://hdl.handle.net/10668/1901
Versión del editor : http://aac.asm.org/content/56/7/3576.abstract
DOI: 10.1128/AAC.06393-11
ISSN : 1098-6596 (Online)
0066-4804 (Print)
Appears in Collections:01- Artículos - Hospital Virgen Macarena

Files in This Item:
File Description SizeFormat 
OrtegaA_SpanishMulticenterStudy.pdfArtículo508,13 kBAdobe PDFView/Open


This item is licensed under a Creative Commons License Creative Commons