Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/1976
Título : CB1 blockade potentiates down-regulation of lipogenic gene expression in perirenal adipose tissue in high carbohydrate diet-induced obesity.
Autor : Vida, Margarita
Rivera, Patricia
Gavito, Ana Luisa
Suárez, Juan
Pavón, Francisco Javier
Arrabal, Sergio
Romero-Cuevas, Miguel
Bautista, Dolores
Martínez, Ana
Rodríguez de Fonseca, Fernando
Serrano, Antonia
Baixeras, Elena
Filiación: [Vida,M; Rivera,P; Gavito,AL; Suárez,J; Pavón,FJ; Arrabal,S; Romero-Cuevas,M; Rodriguez de Fonseca,F; Serrano.A] Unidad de Gestión Clínica de Salud Mental, Hospital Regional Universitario de Málaga, Universidad de Málaga, Málaga, Spain. [Baixeras,E] Unidad de Gestión Clínica de Medicina Interna, Hospital Regional Universitario de Málaga, Universidad de Málaga, Málaga, Spain. [Bautista,D] Unidad de Gestión Clínica de Anatomía Patológica, Hospital Regional Universitario de Málaga, Universidad de Málaga, Málaga, Spain. [Vida,M; Rivera,P; Gavito,AL; Suárez,J; Pavón,FJ; Arrabal,S; Romero-Cuevas,M; Bautista,D; Rodríguez de Fonseca,F; Serrano,A; Baixeras,E] Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Regional Universitario de Málaga, Universidad de Málaga, Málaga, Spain. [Martínez,A] Instituto de Química Médica Lora Tamayo, Consejo Superior de Investigaciones Científicas. Madrid, Spain. [Vida,M; RiveraP; Gavito,AL; Suárez,J; Pavón,FJ; Arrabal,S; Romero-Cuevas,M; Rodríguez de Fonseca;F; Serrano,A] CIBER de Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Santiago de Compostela, Spain.
Palabras clave : Tejido adiposo blanco
Alimentación rica en grasa
Carbohidratos dietéticos
Riñón
Lipogénesis
Peso corporal
Obesidad
MeSH: Medical Subject Headings::Organisms::Eukaryota::Animals
Medical Subject Headings::Phenomena and Processes::Physiological Phenomena::Body Constitution::Body Weights and Measures::Body Size::Body Weight
Medical Subject Headings::Phenomena and Processes::Physiological Phenomena::Nutritional Physiological Phenomena::Diet::Diet, High-Fat
Medical Subject Headings::Technology, Industry, Agriculture::Food and Beverages::Food::Dietary Carbohydrates
Medical Subject Headings::Phenomena and Processes::Physiological Phenomena::Physiological Processes::Pharmacological Processes::Down-Regulation
Medical Subject Headings::Anatomy::Urogenital System::Urinary Tract::Kidney
Medical Subject Headings::Phenomena and Processes::Metabolic Phenomena::Metabolism::Lipid Metabolism::Lipogenesis
Medical Subject Headings::Check Tags::Male
Medical Subject Headings::Phenomena and Processes::Physiological Phenomena::Body Constitution::Body Weights and Measures::Body Size::Body Weight::Overweight::Obesity
Medical Subject Headings::Chemicals and Drugs::Heterocyclic Compounds::Heterocyclic Compounds, 1-Ring::Piperidines
Medical Subject Headings::Chemicals and Drugs::Heterocyclic Compounds::Heterocyclic Compounds, 1-Ring::Azoles::Pyrazoles
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Rats
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Rats::Rats, Wistar
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Membrane Proteins::Receptors, Cell Surface::Receptors, G-Protein-Coupled::Receptors, Cannabinoid::Receptor, Cannabinoid, CB1
Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Signal Transduction
Medical Subject Headings::Phenomena and Processes::Physical Phenomena::Time::Time Factors
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Transcription Factors
Medical Subject Headings::Anatomy::Tissues::Connective Tissue::Adipose Tissue::Adipose Tissue, White
Fecha de publicación : 25-Feb-2014
Editorial : Public Library of Science
Cita Bibliográfica: Vida M, Rivera P, Gavito AL, Suárez J, Pavón FJ, Arrabal S, et al. CB1 blockade potentiates down-regulation of lipogenic gene expression in perirenal adipose tissue in high carbohydrate diet-induced obesity. PLoS ONE. 2014; 9(2):e90016
Abstract: De novo lipogenesis and hypercaloric diets are thought to contribute to increased fat mass, particularly in abdominal fat depots. CB1 is highly expressed in adipose tissue, and CB1-mediated signalling is associated with stimulation of lipogenesis and diet-induced obesity, though its contribution to increasing fat deposition in adipose tissue is controversial. Lipogenesis is regulated by transcription factors such as liver X receptor (LXR), sterol-response element binding protein (SREBP) and carbohydrate-responsive-element-binding protein (ChREBP). We evaluated the role of CB1 in the gene expression of these factors and their target genes in relation to lipogenesis in the perirenal adipose tissue (PrAT) of rats fed a high-carbohydrate diet (HCHD) or a high-fat diet (HFD). Both obesity models showed an up-regulated gene expression of CB1 and Lxrα in this adipose pad. The Srebf-1 and ChREBP gene expressions were down-regulated in HFD but not in HCHD. The expression of their target genes encoding for lipogenic enzymes showed a decrease in diet-induced obesity and was particularly dramatic in HFD. In HCHD, CB1 blockade by AM251 reduced the Srebf-1 and ChREBP expression and totally abrogated the remnant gene expression of their target lipogenic enzymes. The phosphorylated form of the extracellular signal-regulated kinase (ERK-p), which participates in the CB1-mediated signalling pathway, was markedly present in the PrAT of obese rats. ERK-p was drastically repressed by AM251 indicating that CB1 is actually functional in PrAT of obese animals, though its activation loses the ability to stimulate lipogenesis in PrAT of obese rats. Even so, the remnant expression levels of lipogenic transcription factors found in HCHD-fed rats are still dependent on CB1 activity. Hence, in HCHD-induced obesity, CB1 blockade may help to further potentiate the reduction of lipogenesis in PrAT by means of inducing down-regulation of the ChREBP and Srebf-1 gene expression, and consequently in the expression of lipogenic enzymes.
Descripción : Journal Article; Research Support, Non-U.S. Gov't;
URI: http://hdl.handle.net/10668/1976
Versión del editor : http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0090016
DOI: 10.1371/journal.pone.0090016
ISSN : 1932-6203 (Online)
Appears in Collections:01- Artículos - IBIMA. Instituto de Investigación Biomédica de Málaga
01- Artículos - Hospital Regional de Málaga

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