Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/2018
Título : Real-time molecular epidemiology of tuberculosis by direct genotyping of smear-positive clinical specimens.
Autor : Alonso, María
Herranz, Marta
Martínez Lirola, Miguel
González-Rivera, Milagros
Bouza, Emilio
García de Viedma, Darío
Filiación: [Alonso,M; Herranz,M; Bouza,E; García de Viedma,D] Hosp Gen Univ Gregorio Marañon, Serv Microbiol & Enfermedades Infecciosas, Madrid, Spain. [Alonso,M; Herranz,M; Bouza, E; García de Viedma,D; González-Rivera,M ] Inst Invest Sanitaria Gregorio Marañon, Madrid, Spain. [Alonso,M; Herranz,M; Bouza,E; García de Viedma,D] CIBER Enfermedades Respiratorias. CIBERES.Spain.[Martínez Lirola,M] Complejo Hosp Torrecárdenas,Almería,Spain.
Palabras clave : ADN bacteriano
Tipificación molecular
Esputo
Tuberculosis pulmonar
MeSH: Medical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::DNA::DNA, Bacterial
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Molecular Typing
Medical Subject Headings::Organisms::Bacteria::Gram-Positive Bacteria::Gram-Positive Rods::Gram-Positive Asporogenous Rods::Gram-Positive Asporogenous Rods, Regular::Mycobacteriaceae::Mycobacterium::Mycobacterium tuberculosis
Medical Subject Headings::Diseases::Respiratory Tract Diseases::Respiratory Tract Infections::Tuberculosis, Pulmonary
Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Molecular Structure::Base Sequence::Repetitive Sequences, Nucleic Acid::Tandem Repeat Sequences::Minisatellite Repeats
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Molecular Epidemiology
Medical Subject Headings::Anatomy::Fluids and Secretions::Bodily Secretions::Sputum
Fecha de publicación : May-2012
Editorial : American Society for Microbiology
Cita Bibliográfica: Alonso M, Herranz M, Martínez Lirola M, González-Rivera M, Bouza E, García de Viedma D. Real-time molecular epidemiology of tuberculosis by direct genotyping of smear-positive clinical specimens. J. Clin. Microbiol.. 2012 May; 50(5):1755-7
Abstract: We applied MIRU-VNTR (mycobacterial interspersed repetitive-unit-variable-number tandem-repeat typing) to directly analyze the bacilli present in 61 stain-positive specimens from tuberculosis patients. A complete MIRU type (24 loci) was obtained for all but one (no amplification in one locus) of the specimens (98.4%), and the allelic values fully correlated with those obtained from the corresponding cultures. Our study is the first to demonstrate that real-time genotyping of Mycobacterium tuberculosis can be achieved, fully transforming the way in which molecular epidemiology techniques can be integrated into control programs.
Descripción : Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't;
URI: http://hdl.handle.net/10668/2018
Versión del editor : http://jcm.asm.org/content/50/5/1755.long
DOI: 10.1128/JCM.00132-12
ISSN : 1098-660X(Online)
0095-1137
Appears in Collections:01- Artículos - Complejo Hospitalario Torrecárdenas

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