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http://hdl.handle.net/10668/2018| Title: | Real-time molecular epidemiology of tuberculosis by direct genotyping of smear-positive clinical specimens. |
| Authors: | Alonso, María Herranz, Marta Martínez Lirola, Miguel González-Rivera, Milagros Bouza, Emilio García de Viedma, Darío |
| metadata.dc.contributor.authoraffiliation: | [Alonso,M; Herranz,M; Bouza,E; García de Viedma,D] Hosp Gen Univ Gregorio Marañon, Serv Microbiol & Enfermedades Infecciosas, Madrid, Spain. [Alonso,M; Herranz,M; Bouza, E; García de Viedma,D; González-Rivera,M ] Inst Invest Sanitaria Gregorio Marañon, Madrid, Spain. [Alonso,M; Herranz,M; Bouza,E; García de Viedma,D] CIBER Enfermedades Respiratorias. CIBERES.Spain.[Martínez Lirola,M] Complejo Hosp Torrecárdenas,Almería,Spain. |
| Keywords: | ADN bacteriano;Tipificación molecular;Esputo;Tuberculosis pulmonar |
| metadata.dc.subject.mesh: | Medical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::DNA::DNA, Bacterial Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Molecular Typing Medical Subject Headings::Organisms::Bacteria::Gram-Positive Bacteria::Gram-Positive Rods::Gram-Positive Asporogenous Rods::Gram-Positive Asporogenous Rods, Regular::Mycobacteriaceae::Mycobacterium::Mycobacterium tuberculosis Medical Subject Headings::Diseases::Respiratory Tract Diseases::Respiratory Tract Infections::Tuberculosis, Pulmonary Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Molecular Structure::Base Sequence::Repetitive Sequences, Nucleic Acid::Tandem Repeat Sequences::Minisatellite Repeats Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Molecular Epidemiology Medical Subject Headings::Anatomy::Fluids and Secretions::Bodily Secretions::Sputum |
| Issue Date: | May-2012 |
| Publisher: | American Society for Microbiology |
| Citation: | Alonso M, Herranz M, Martínez Lirola M, González-Rivera M, Bouza E, García de Viedma D. Real-time molecular epidemiology of tuberculosis by direct genotyping of smear-positive clinical specimens. J. Clin. Microbiol.. 2012 May; 50(5):1755-7 |
| Abstract: | We applied MIRU-VNTR (mycobacterial interspersed repetitive-unit-variable-number tandem-repeat typing) to directly analyze the bacilli present in 61 stain-positive specimens from tuberculosis patients. A complete MIRU type (24 loci) was obtained for all but one (no amplification in one locus) of the specimens (98.4%), and the allelic values fully correlated with those obtained from the corresponding cultures. Our study is the first to demonstrate that real-time genotyping of Mycobacterium tuberculosis can be achieved, fully transforming the way in which molecular epidemiology techniques can be integrated into control programs. |
| Description: | Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't; |
| URI: | http://hdl.handle.net/10668/2018 |
| metadata.dc.relation.publisherversion: | http://jcm.asm.org/content/50/5/1755.long |
| metadata.dc.identifier.doi: | 10.1128/JCM.00132-12 |
| ISSN: | 1098-660X(Online) 0095-1137 |
| Appears in Collections: | 01- Artículos - Complejo Hospitalario Torrecárdenas |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Alonso_RealTime.pdf | Artículo publicado | 172,67 kB | Adobe PDF | View/Open |
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