Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/2036
Título : UV-fixed-thick-blotch preparation improves sensitivity of auramine staining.
Autor : Cejudo-García, M A
Muñoz-Davila, M J
Martínez-Lirola, M J
Filiación: [Cejudo-García,MA; Muñoz-Davila,MJ; Martínez-Lirola,MJ] Microbiology Laboratory, Biotechnology Clinic Management Unit, Torrecárdenas Hospital Complex, Almería, Spain
Palabras clave : Tuberculosis
Mycobacterium
Técnicas bacteriológicas
Benzofenoneido
Esputo
Manejo de especímenes
MeSH: Medical Subject Headings::Diseases::Bacterial Infections and Mycoses::Bacterial Infections::Gram-Positive Bacterial Infections::Actinomycetales Infections::Mycobacterium Infections::Tuberculosis
Medical Subject Headings::Organisms::Bacteria::Gram-Positive Bacteria::Gram-Positive Rods::Gram-Positive Asporogenous Rods::Gram-Positive Asporogenous Rods, Regular::Mycobacteriaceae::Mycobacterium
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Microbiological Techniques::Bacteriological Techniques
Medical Subject Headings::Chemicals and Drugs::Organic Chemicals::Amines::Aniline Compounds::Benzophenoneidum
Medical Subject Headings::Anatomy::Fluids and Secretions::Bodily Secretions::Sputum
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Specimen Handling
Fecha de publicación : Oct-2013
Editorial : American Society for Microbiology
Cita Bibliográfica: Cejudo-García MA, Muñoz-Davila MJ, Martínez-Lirola MJ. UV-fixed-thick-blotch preparation improves sensitivity of auramine staining. J. Clin. Microbiol.. 2013 ; 51(10):3400-2
Abstract: We describe here a very simple modification of the auramine staining procedure based on preparation of a UV-fixed thick blotch which allowed us to reach an overall sensitivity of 0.82 (592 acid-fast bacillus [AFB]-positive specimens/722 initial respiratory specimens with positive mycobacterial culture) and sensitivities of 0.93 (526 AFB-positive specimens/564 culture-positive specimens) for Mycobacterium tuberculosis complex and 0.42 (66 AFB-positive specimens/158 culture-positive specimens) for nontuberculous mycobacteria.
URI: http://hdl.handle.net/10668/2036
Versión del editor : http://jcm.asm.org/content/51/10/3400.abstract
DOI: 10.1128/JCM.00974-13
ISSN : 1098-660X (Online)
0095-1137 (Print)
Appears in Collections:01- Artículos - Complejo Hospitalario Torrecárdenas

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