Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/2055
Título : Analysis of genes encoding penicillin-binding proteins in clinical isolates of Acinetobacter baumannii.
Autor : Cayô, Rodrigo
Rodríguez, María-Cruz
Espinal, Paula
Fernández-Cuenca, Felipe
Ocampo-Sosa, Alain A
Pascual, Alvaro
Ayala, Juan A
Vila, Jordi
Martínez-Martínez, Luis
Filiación: [Cayô,R; Rodríguez,MC; Ocampo-Sosa,AA; Martínez-Martínez,L] Service of Microbiology, University Hospital Marqués de Valdecilla—IFIMAV [Espinal,P; Vila,J] Service of Microbiology, Hospital Clinic, School of Medicine, University of Barcelona, Barcelona. [Fernández-Cuenca,F; Pascual,A] Service of Microbiology, University Hospital Virgen Macarena, Seville. [Ayala,JA] Centro de Biología Molecular Severo Ochoa—CSIC-UAM, Campus de Cantoblanco, Madrid, Spain. [Rodríguez,MC; Martínez-Martínez,L] Department of Molecular Biology, School of Medicine, University of Cantabria, Santander.
Palabras clave : Secuencia de Aminoácidos
Hospitales Escuela
Pruebas de Sensibilidad Microbiana
Proteínas de Unión a las Penicilinas
Análisis de Secuencia de ADN
Resistencia beta-Lactámica
MeSH: Medical Subject Headings::Organisms::Bacteria::Gram-Negative Bacteria::Gram-Negative Aerobic Bacteria::Gram-Negative Aerobic Rods and Cocci::Moraxellaceae::Acinetobacter::Acinetobacter baumannii
Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Molecular Structure::Amino Acid Sequence
Medical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Therapeutic Uses::Anti-Infective Agents::Anti-Bacterial Agents
Medical Subject Headings::Chemicals and Drugs::Organic Chemicals::Amides::Lactams::beta-Lactams::Carbapenems
Medical Subject Headings::Health Care::Health Care Facilities, Manpower, and Services::Health Facilities::Academic Medical Centers::Hospitals, Teaching
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Microbiological Techniques::Microbial Sensitivity Tests
Medical Subject Headings::Information Science::Information Science::Information Services::Documentation::Molecular Sequence Data
Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Penicillin-Binding Proteins
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Variation::Mutation::Point Mutation
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Sequence Analysis::Sequence Analysis, DNA
Medical Subject Headings::Geographicals::Geographic Locations::Europe::Spain
Medical Subject Headings::Phenomena and Processes::Microbiological Phenomena::Bacterial Physiological Phenomena::Drug Resistance, Bacterial::beta-Lactam Resistance
Medical Subject Headings::Diseases::Bacterial Infections and Mycoses::Bacterial Infections::Gram-Negative Bacterial Infections::Moraxellaceae Infections::Acinetobacter Infections
Fecha de publicación : Dec-2011
Editorial : American Society for Microbiology
Cita Bibliográfica: Cayô R, Rodríguez MC, Espinal P, Fernández-Cuenca F, Ocampo-Sosa AA, Pascual A, et al. Analysis of genes encoding penicillin-binding proteins in clinical isolates of Acinetobacter baumannii. Antimicrob. Agents Chemother. 2011; 55(12):5907-13
Abstract: There is limited information on the role of penicillin-binding proteins (PBPs) in the resistance of Acinetobacter baumannii to β-lactams. This study presents an analysis of the allelic variations of PBP genes in A. baumannii isolates. Twenty-six A. baumannii clinical isolates (susceptible or resistant to carbapenems) from three teaching hospitals in Spain were included. The antimicrobial susceptibility profile, clonal pattern, and genomic species identification were also evaluated. Based on the six complete genomes of A. baumannii, the PBP genes were identified, and primers were designed for each gene. The nucleotide sequences of the genes identified that encode PBPs and the corresponding amino acid sequences were compared with those of ATCC 17978. Seven PBP genes and one monofunctional transglycosylase (MGT) gene were identified in the six genomes, encoding (i) four high-molecular-mass proteins (two of class A, PBP1a [ponA] and PBP1b [mrcB], and two of class B, PBP2 [pbpA or mrdA] and PBP3 [ftsI]), (ii) three low-molecular-mass proteins (two of type 5, PBP5/6 [dacC] and PBP6b [dacD], and one of type 7 (PBP7/8 [pbpG]), and (iii) a monofunctional enzyme (MtgA [mtgA]). Hot spot mutation regions were observed, although most of the allelic changes found translated into silent mutations. The amino acid consensus sequences corresponding to the PBP genes in the genomes and the clinical isolates were highly conserved. The changes found in amino acid sequences were associated with concrete clonal patterns but were not directly related to susceptibility or resistance to β-lactams. An insertion sequence disrupting the gene encoding PBP6b was identified in an endemic carbapenem-resistant clone in one of the participant hospitals.
Descripción : Journal Article; Research Support, Non-U.S. Gov't;
URI: http://hdl.handle.net/10668/2055
Versión del editor : http://aac.asm.org/content/55/12/5907.abstract
DOI: 10.1128/AAC.00459-11
ISSN : 1098-6596 (Online)
0066-4804 (Print)
Appears in Collections:01- Artículos - Hospital Virgen Macarena

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