Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/2072
Título : Zinc-α2-Glycoprotein Modulates AKT-Dependent Insulin Signaling in Human Adipocytes by Activation of the PP2A Phosphatase.
Autor : Ceperuelo-Mallafré, Victoria
Ejarque, Miriam
Durán, Xavier
Pachón, Gisela
Vázquez-Carballo, Ana
Roche, Kelly
Núñez-Roa, Catalina
Garrido-Sánchez, Lourdes
Tinahones, Francisco J
Vendrell, Joan
Fernández-Veledo, Sonia
Filiación: [Ceperuelo-Mallafré,V; Ejarque,M; Pachón,G; Roche,K; Núñez-Roa,C; Vendrell,J; Fernández-Veledo,S] Hospital Universitari de Tarragona Joan XXIII, Institut d'Investigació Sanitària Pere Virgili, Universitat Rovira I Virgili, Tarragona, Spain. [Ceperuelo-Mallafré,V; Ejarque,M; Durán,X; Pachón,G; Roche,K; Núñez-Roa,C; Vendrell,J; Fernández-Veledo,S] Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabolicas Asociadas, Instituto de Salud Carlos III, Madrid, Spain. [Vázquez-Carballo,A] Departament of Biochemistry and Molecular Biology II, School of Pharmacy, Complutense University, Madrid, Spain. [Garrido-Sánchez,L; Tinahones,FJ] Hospital Universitario Virgen de la Victoria, Instituto de Investigaciones Biomédicas de Málaga, Spain. [Garrido-Sánchez,L; Tinahones,FJ] Centro de Investigación Biomédica en Red de Fisiopatología de la Obesidad y la Nutrición, Instituto de Salud Carlos III, Madrid, Spain.
Palabras clave : Fosfoproteína fosfatasas
Glucosa
Resistencia a la insulina
Activación enzimática
Transducción de señal
Adipocitos marrones
MeSH: Medical Subject Headings::Chemicals and Drugs::Hormones, Hormone Substitutes, and Hormone Antagonists::Hormones::Peptide Hormones::Pancreatic Hormones::Insulins
Medical Subject Headings::Anatomy::Cells::Connective Tissue Cells::Adipocytes
Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Hydrolases::Esterases::Phosphoric Monoester Hydrolases::Phosphoprotein Phosphatases
Medical Subject Headings::Chemicals and Drugs::Carbohydrates::Monosaccharides::Hexoses::Glucose
Medical Subject Headings::Diseases::Nutritional and Metabolic Diseases::Metabolic Diseases::Glucose Metabolism Disorders::Hyperinsulinism::Insulin Resistance
Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Biochemical Processes::Enzyme Activation
Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Biochemical Processes::Signal Transduction
Medical Subject Headings::Anatomy::Cells::Connective Tissue Cells::Adipocytes::Adipocytes, Brown
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Fecha de publicación : 11-Jun-2015
Editorial : Public Library of Science
Cita Bibliográfica: Ceperuelo-Mallafré V, Ejarque M, Duran X, Pachón G, Vázquez-Carballo A, Roche K, et al. Zinc-α2-Glycoprotein Modulates AKT-Dependent Insulin Signaling in Human Adipocytes by Activation of the PP2A Phosphatase. PLoS ONE 2015; 10(6):e0129644
Abstract: OBJECTIVE Evidence from mouse models suggests that zinc-α2-glycoprotein (ZAG) is a novel anti-obesity adipokine. In humans, however, data are controversial and its physiological role in adipose tissue (AT) remains unknown. Here we explored the molecular mechanisms by which ZAG regulates carbohydrate metabolism in human adipocytes. METHODS ZAG action on glucose uptake and insulin action was analyzed. β1 and β2-adrenoreceptor (AR) antagonists and siRNA targeting PP2A phosphatase were used to examine the mechanisms by which ZAG modulates insulin sensitivity. Plasma levels of ZAG were measured in a lean patient cohort stratified for HOMA-IR. RESULTS ZAG treatment increased basal glucose uptake, correlating with an increase in GLUT expression, but induced insulin resistance in adipocytes. Pretreatment of adipocytes with propranolol and a specific β1-AR antagonist demonstrated that ZAG effects on basal glucose uptake and GLUT4 expression are mediated via β1-AR, whereas inhibition of insulin action is dependent on β2-AR activation. ZAG treatment correlated with an increase in PP2A activity. Silencing of the PP2A catalytic subunit abrogated the negative effect of ZAG on insulin-stimulated AKT phosphorylation and glucose uptake but not on GLUT4 expression and basal glucose uptake. ZAG circulating levels were unchanged in a lean patient cohort stratified for HOMA-IR. Neither glucose nor insulin was associated with plasma ZAG. CONCLUSIONS ZAG inhibits insulin-induced glucose uptake in human adipocytes by impairing insulin signaling at the level of AKT in a β2-AR- and PP2A-dependent manner.
Descripción : Journal Article;
URI: http://hdl.handle.net/10668/2072
Versión del editor : http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0129644#abstract0
DOI: 10.1371/journal.pone.0129644
ISSN : 1932-6203 (Online)
Appears in Collections:01- Artículos - IBIMA. Instituto de Investigación Biomédica de Málaga
01- Artículos - Hospital Virgen de la Victoria

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