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Título : Neutrophil defensins: their possible role in allergic asthma.
Autor : Vega, A
Ventura, I
Chamorro, C
Aroca, R
Orovigt, A
Gómez, E
Puente, Y
Martínez, A
Asturias, J A
Monteseirín, J
Filiación: [Vega,A; Ventura,I; Chamorro,C; Aroca,R; Orovigt,A; Gómez,E; Puente,Y; Monteseirín,J] Servicio Regional de Inmunología y Alergia, Hospital Universitario Virgen Macarena, Facultad de Medicina, Universidad de Sevilla, Sevilla, Spain. [Martínez,A; Asturias,JA] Bial-Aristegui, R&D Department, Bilbao, Spain.
Palabras clave : Alpha-Defensins
Allergen challenge
Células Cultivadas
MeSH: Medical Subject Headings::Chemicals and Drugs::Biological Factors::Antigens::Allergens
Medical Subject Headings::Diseases::Respiratory Tract Diseases::Bronchial Diseases::Asthma
Medical Subject Headings::Anatomy::Cells::Cells, Cultured
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Anatomy::Cells::Blood Cells::Leukocytes::Granulocytes::Neutrophils
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Peptides::Antimicrobial Cationic Peptides::Defensins::alpha-Defensins
Medical Subject Headings::Named Groups::Persons::Age Groups::Adult
Fecha de publicación : 2011
Editorial : ESMON Publicidad
Cita Bibliográfica: Vega A, Ventura I, Chamorro C, Aroca R, Orovigt A, Gómez E, et al. Neutrophil defensins: their possible role in allergic asthma. J Investig Allergol Clin Immunol. 2011; 21(1):38-43
Abstract: BACKGROUND Neutrophil defensins, originally identified as broad-spectrum antimicrobial peptides, have been implicated in the regulation of inflammatory and immunological processes. OBJECTIVES To investigate whether the in vitro challenge of neutrophils from patients with bronchial asthma with allergens stimulated the release of alpha-defensins and whether levels released were dependent on lung infections. METHOD The neutrophils were cultivated with different agonists and the concentration of alpha-defensin in cell-free supernatant was measured with enzyme-linked immunosorbent assay (ELISA). RESULTS Neutrophils from allergic patients released alpha-defensins via an allergen-dependent mechanism. Our results indicate that the in vitro activation of neutrophils is highly allergen-specific. In this context, allergens other than those which produced clinical symptoms did not elicit alpha-defensin release, and allergens had no effect on neutrophils from healthy donors. However, neutrophils from both allergic patients and healthy controls were able to release alpha-defensins upon treatment with PMA. In the allergen-stimulated neutrophils, cells from asthmatic patients stimulated with a sensitizing allergen showed a significantly higher production of alpha-defensin under respiratory tract infection than cells from the same patients without such an infection. CONCLUSION Neutrophils from allergic patients release alpha-defensins via an allergen-dependent mechanism.
Descripción : Journal Article; Research Support, Non-U.S. Gov't;
Versión del editor :
ISSN : 1018-9068 (Online)
Appears in Collections:01- Artículos - Hospital Virgen Macarena

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