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Title: Allergens Induce the Release of Lactoferrin by Neutrophils from Asthmatic Patients.
Authors: Fernández-Delgado, Lourdes
Vega-Rioja, Antonio
Ventura, Inmaculada
Chamorro, Cristina
Aroca, Rocío
Prados, Manuel
Bobadilla, Pedro
Rodríguez, David
Palacios, Ricardo
Monteseirín, Javier
metadata.dc.contributor.authoraffiliation: [Fernández-Delgado,L; Vega-Rioja,A; Ventura,I; Chamorro,C; Aroca,R; Prados,M; Monteseirín,J] Unidad de Gestión Clínica de Alergia Intercentros, Hospital Universitario Virgen Macarena, Sevilla, Spain. [Monteseirín,J] Departamento de Medicina. Facultad de Medicina. Universidad de Sevilla, Sevilla, Spain. [Bobadilla,P] Servicio de Alergia, Hospital Infanta Elena, Badajoz, Spain. [Rodríguez,D; Palacios,R] Laboratorios Diater, Madrid, Spain.
Keywords: Alérgenos;Anticuerpos monoclonales;Asma;Galectina 3;Inmunosupresores
metadata.dc.subject.mesh: Medical Subject Headings::Chemicals and Drugs::Biological Factors::Antigens::Allergens
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Blood Proteins::Immunoproteins::Immunoglobulins::Antibodies::Antibodies, Monoclonal
Medical Subject Headings::Diseases::Respiratory Tract Diseases::Lung Diseases::Lung Diseases, Obstructive::Asthma
Medical Subject Headings::Phenomena and Processes::Biological Phenomena::Biological Processes
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Lectins::Galectins::Galectin 3
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Blood Proteins::Immunoproteins::Immunoglobulins::Antibodies::Immunoglobulin Isotypes::Immunoglobulin E
Medical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Physiological Effects of Drugs::Immunologic Factors::Immunosuppressive Agents
Medical Subject Headings::Chemicals and Drugs::Biological Factors::Inflammation Mediators
Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Hydrolases::Peptide Hydrolases::Endopeptidases::Serine Endopeptidases::Lactoferrin
Medical Subject Headings::Anatomy::Cells::Blood Cells::Leukocytes::Granulocytes::Neutrophils
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Membrane Proteins::Receptors, Cell Surface::Receptors, Immunologic::Receptors, Fc::Receptors, IgE
Issue Date: 21-Oct-2015
Publisher: Public Library of Science
Citation: Fernández-Delgado L, Vega-Rioja A, Ventura I, Chamorro C, Aroca R, Prados M, et al. Allergens Induce the Release of Lactoferrin by Neutrophils from Asthmatic Patients. PLoS ONE. 2015; 10(10):e0141278
Abstract: BACKGROUND Despite the evidence that Lactoferrin (Lf) is involved in allergic asthma processes, it is unknown whether neutrophils can be one of the main cellular sources of this key inflammatory mediator directly in response of an IgE mediated stimulus. The present study was undertaken to analyze this question. METHODS Neutrophils from healthy subjects (n = 34) and neutrophils from allergic asthmatic patients (n = 102) were challenged in vitro with specific allergens to which the patients were sensitized, PAF, or agonist mAbs against IgE-receptors, and the levels of Lf were measured in the culture supernatant. The levels of serum IgE together with the severity of symptoms were also analyzed. RESULTS Lf was released into the culture supernatant of neutrophils from allergic asthmatic patients in response to allergens and PAF. This response was highly allergen-specific, and did not happen in neutrophils from healthy donors. Allergen effect was mimicked by Abs against FcεRI and galectin-3 but not by FcεRII. The levels of released Lf correlated well with the levels of serum specific IgE and severity of asthma symptoms. These observations represent a novel view of neutrophils as an important source of Lf in allergic asthma. Importantly, the levels of released Lf by neutrophils could therefore be used to evaluate disease severity in allergic asthmatic patients.
Description: Journal Article;
metadata.dc.identifier.doi: 10.1371/journal.pone.0141278
ISSN: 1932-6203 (Online)
Appears in Collections:01- Artículos - Hospital Virgen Macarena

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