Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/2122
Title: Specimen storage in transport medium and detection of group B streptococci by culture
Authors: de la Rosa-Fraile, Manuel
Camacho-Muñoz, Enrique
Rodríguez-Granger, Javier
Liébana-Martos, Carmen
metadata.dc.contributor.authoraffiliation: Microbiology Service, Virgen de las Nieves University Hospital, Granada, Spain.
Keywords: Técnicas bacteriológicas;Medios de cultivo;Femenino;Humanos;Embarazo;Complicaciones infecciosas del embarazo;Recto;Manejo de especímenes;Infecciones estreptocócicas;Streptococcus agalactiae
metadata.dc.subject.mesh: Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Clinical Laboratory Techniques::Microbiological Techniques::Bacteriological Techniques
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Equipment and Supplies::Culture Media
Medical Subject Headings::Check Tags::Female
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Phenomena and Processes::Reproductive and Urinary Physiological Phenomena::Reproductive Physiological Phenomena::Reproductive Physiological Processes::Reproduction::Pregnancy
Medical Subject Headings::Diseases::Female Urogenital Diseases and Pregnancy Complications::Pregnancy Complications::Pregnancy Complications, Infectious
Medical Subject Headings::Anatomy::Digestive System::Gastrointestinal Tract::Intestines::Intestine, Large::Rectum
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Clinical Laboratory Techniques::Specimen Handling
Medical Subject Headings::Diseases::Bacterial Infections and Mycoses::Bacterial Infections::Gram-Positive Bacterial Infections::Streptococcal Infections
Medical Subject Headings::Organisms::Bacteria::Gram-Positive Bacteria::Gram-Positive Cocci::Streptococcaceae::Streptococcus::Streptococcus agalactiae
Medical Subject Headings::Anatomy::Urogenital System::Genitalia::Genitalia, Female::Vagina
Issue Date: Feb-2005
Publisher: American Society for Microbiology
Citation: de la Rosa-Fraile M , Camacho-Muñoz E, Rodríguez-Granger J, Liébana-Martos C. Specimen storage in transport medium and detection of group B streptococci by culture. J Clin Microbiol. 2005 Feb;43(2):928-30.
Abstract: Recovery of group B streptococci (GBS) was assessed in 1,204 vaginorectal swabs stored in Amies transport medium at 4 or 21°C for 1 to 4 days either by direct inoculation onto Granada agar (GA) or by culture in blood agar (BA) and GA after a selective broth enrichment (SBE) step. Following storage at 4°C, GBS detection in GA was not affected after 72 h by either direct inoculation or SBE; however, GBS were not detected after SBE in the BA subculture in some samples after 48 h of storage and in GA after 96 h. After storage at 21°C, loss of GBS-positive results was significant after 48 h by direct inoculation in GA and after 96 h by SBE and BA subculture; some GBS-positive samples were not detected after 24 h of storage followed by SBE and BA subculture or after 48 h of storage followed by SBE and GA subculture. Storage of swabs in transport medium, even at 4°C, produced after 24 h an underestimation of the intensity of GBS colonization in most specimens. These data indicate that viability of GBS is not fully preserved by storage of vaginorectal swabs in Amies transport medium, mainly if they are not stored under refrigeration
URI: http://hdl.handle.net/10668/2122
metadata.dc.relation.publisherversion: http://jcm.asm.org/content/43/2/928.abstract
metadata.dc.identifier.doi: 10.1128/​JCM.43.2.928-930.2005
ISSN: 0095-1137 (Print)
1098-660X (Online)
Appears in Collections:01- Artículos - Hospital Virgen de las Nieves

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