Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/2123
Título : Use of Granada medium to detect group B streptococcal colonization in pregnant women.
Autor : Rosa-Fraile, Manuel
Rodriguez-Granger, Javier
Cueto-Lopez, Marina
Sampedro, Antonio
Biel Gaye, Enrique
Haro, José Manuel
Andreu, Antonia
Filiación: [Rosa-Fraile,M; Rodriguez-Granger,J; Cueto-Lopez,M; Sampedro,A] Microbiology Service, Virgen de las Nieves Hospital, Granada, Spain. [Biel Gaye,E; Haro,JM] Obstetric & Gynecology Department, Virgen de las Nieves Hospital, Granada, Spain. [Andreu,A] Microbiology Service, Vall D’Hebron Hospital, Barcelona, Spain.
Palabras clave : Medios de cultivo
Embarazo
Complicaciones infecciosas del embarazo
Estudios prospectivos
Infecciones estreptocócicas
Técnicas de tipificación bacteriana
Granada
España
MeSH: Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Equipment and Supplies::Culture Media
Medical Subject Headings::Check Tags::Female
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Phenomena and Processes::Reproductive and Urinary Physiological Phenomena::Reproductive Physiological Phenomena::Reproductive Physiological Processes::Reproduction::Pregnancy
Medical Subject Headings::Diseases::Female Urogenital Diseases and Pregnancy Complications::Pregnancy Complications::Pregnancy Complications, Infectious
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Epidemiologic Study Characteristics as Topic::Epidemiologic Studies::Cohort Studies::Longitudinal Studies::Prospective Studies
Medical Subject Headings::Diseases::Bacterial Infections and Mycoses::Bacterial Infections::Gram-Positive Bacterial Infections::Streptococcal Infections
Medical Subject Headings::Organisms::Bacteria::Gram-Positive Bacteria::Gram-Positive Cocci::Streptococcaceae::Streptococcus
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Clinical Laboratory Techniques::Microbiological Techniques::Bacteriological Techniques::Bacterial Typing Techniques
Fecha de publicación : Aug-1999
Editorial : American Society for Microbiology
Cita Bibliográfica: Rosa-Fraile M, Rodriguez-Granger J, Cueto-Lopez M, Sampedro A, Biel Gaye E, Haro JM, et al. Use of Granada medium to detect group B streptococcal colonization in pregnant women. J Clin Microbiol. 1999; 37(8):2674-7.
Abstract: Direct inoculation onto Granada medium (GM) in plates and tubes was compared to inoculation into a selective Todd-Hewitt broth (with 8 microg of gentamicin per ml and 15 microg of nalidixic acid per ml) for detection of group B streptococci (GBS) in pregnant women with 800 vaginal and 450 vaginoanorectal samples. Comparatively, GM was found to be as sensitive as the selective broth for the detection of GBS in vaginal specimens and more sensitive than selective broth for the detection of GBS in vaginoanorectal samples (96 versus 82%). The use of GM improved the time to reporting of a GBS-positive result by at least 24 h and reduced the direct cost of screening. We have also found that the inconvenience of anaerobic incubation of GM plates can be avoided when a cover slide is placed upon the inoculum, because aerobic incubation in GM plates with cover slides causes GBS to develop the same pigmentation that it develops with incubation under anaerobic conditions. These data support the routine use of GM plates or tubes as a more accurate, easier, and cheaper method of identification of GBS-colonized women compared to the enrichment broth technique.
Descripción : Journal Article;
URI: http://hdl.handle.net/10668/2123
Versión del editor : http://jcm.asm.org/content/37/8/2674.abstract
ISSN : 1098-660X (Online)
0095-1137 (Print)
Appears in Collections:01- Artículos - Hospital Virgen de las Nieves

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