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Title: Diagnostic yield of a PCR assay in focal complications of brucellosis.
Authors: Morata, P.
Queipo-Ortuño, M. I.
Reguera, J. M.
Miralles, F.
Lopez-Gonzalez, J. J.
Colmenero, J. D.
metadata.dc.contributor.authoraffiliation: [Morata,P; Queipo-Ortuño,I] Department of Biochemistry and Molecular Biology, Faculty of Medicine, Málaga University. [Reguera,JM; Miralles,F; Colmenero,JD] Infectious Diseases Unit, Internal Medicine Department, “Carlos Haya” University Hospital, Málaga, Spain. [Lopez-Gonzalez,JJ] Microbiology Service, “Carlos Haya” University Hospital, Málaga, Spain.
Keywords: Brucela;Brucelosis;Medios de cultivo;ADN bacteriano;Reacción en cadena de la polimerasa;Sensibilidad y especificidad
metadata.dc.subject.mesh: Medical Subject Headings::Named Groups::Persons::Age Groups::Adult::Aged
Medical Subject Headings::Named Groups::Persons::Age Groups::Adult::Aged::Aged, 80 and over
Medical Subject Headings::Organisms::Bacteria::Gram-Negative Bacteria::Gram-Negative Aerobic Bacteria::Gram-Negative Aerobic Rods and Cocci::Brucellaceae::Brucella
Medical Subject Headings::Diseases::Bacterial Infections and Mycoses::Bacterial Infections::Gram-Negative Bacterial Infections::Brucellosis
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Equipment and Supplies::Culture Media
Medical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::DNA::DNA, Bacterial
Medical Subject Headings::Check Tags::Female
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Check Tags::Male
Medical Subject Headings::Named Groups::Persons::Age Groups::Adult::Middle Aged
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Nucleic Acid Amplification Techniques::Polymerase Chain Reaction
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Statistics as Topic::Sensitivity and Specificity
Medical Subject Headings::Named Groups::Persons::Age Groups::Adult
Issue Date: Oct-2001
Publisher: American Society for Microbiology
Citation: Morata P, Queipo-Ortuño MI, Reguera JM, Miralles F, Lopez-Gonzalez JJ, Colmenero JD. Diagnostic yield of a PCR assay in focal complications of brucellosis. J Clin Microbiol. 2001; 39(10):3743-6
Abstract: In order to evaluate the diagnostic yield of a PCR assay for patients with focal complications of brucellosis, we studied by PCR and by conventional microbiological techniques 34 nonblood samples from 32 patients with different focal forms of brucellosis. The samples from patients with brucellosis were paired to an equal number of control samples from the same locations of patients whose illnesses had different etiologies. Thirty-three of the 34 nonblood samples (97%) from the brucellosis patients were positive by PCR, whereas Brucella spp. were isolated from only 29.4% of the conventional cultures. For 11.4% of the patients, the confirmatory serological tests were either negative or showed titers below the diagnostic range. Two patients (6.2%) from the control group, both with tuberculous vertebral osteomyelitis, had a positive PCR result. The brucella PCR of blood from these two patients was also positive, and the two strains of Mycobacterium tuberculosis isolated were analyzed by the brucella PCR, with no evidence of amplification. These results show that the PCR assay is far more sensitive than conventional cultures, and this, coupled with its speed and reduction in risk to laboratory workers, makes this technique a very useful tool for the diagnosis of focal complications of brucellosis.
Description: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't;
metadata.dc.identifier.doi: 10.1128/JCM.39.10.3743-3746.2001
ISSN: 0095-1137 (Online)
Appears in Collections:01- Artículos - Hospital Regional de Málaga

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