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dc.contributor.authorMorata, P.-
dc.contributor.authorQueipo-Ortuño, M. I.-
dc.contributor.authorReguera, J. M.-
dc.contributor.authorMiralles, F.-
dc.contributor.authorLopez-Gonzalez, J. J.-
dc.contributor.authorColmenero, J. D.-
dc.identifier.citationMorata P, Queipo-Ortuño MI, Reguera JM, Miralles F, Lopez-Gonzalez JJ, Colmenero JD. Diagnostic yield of a PCR assay in focal complications of brucellosis. J Clin Microbiol. 2001; 39(10):3743-6es
dc.identifier.issn0095-1137 (Online)-
dc.descriptionEvaluation Studies; Journal Article; Research Support, Non-U.S. Gov't;es
dc.description.abstractIn order to evaluate the diagnostic yield of a PCR assay for patients with focal complications of brucellosis, we studied by PCR and by conventional microbiological techniques 34 nonblood samples from 32 patients with different focal forms of brucellosis. The samples from patients with brucellosis were paired to an equal number of control samples from the same locations of patients whose illnesses had different etiologies. Thirty-three of the 34 nonblood samples (97%) from the brucellosis patients were positive by PCR, whereas Brucella spp. were isolated from only 29.4% of the conventional cultures. For 11.4% of the patients, the confirmatory serological tests were either negative or showed titers below the diagnostic range. Two patients (6.2%) from the control group, both with tuberculous vertebral osteomyelitis, had a positive PCR result. The brucella PCR of blood from these two patients was also positive, and the two strains of Mycobacterium tuberculosis isolated were analyzed by the brucella PCR, with no evidence of amplification. These results show that the PCR assay is far more sensitive than conventional cultures, and this, coupled with its speed and reduction in risk to laboratory workers, makes this technique a very useful tool for the diagnosis of focal complications of
dc.description.sponsorshipThis work received financial support from the Inter-Ministerial Commission for Science and Technology (CICYT) and the European Commission (grant IFD97-0539).es
dc.publisherAmerican Society for Microbiologyes
dc.relation.ispartofJournal of Clinical Microbiologyes
dc.subjectMedios de cultivoes
dc.subjectADN bacterianoes
dc.subjectReacción en cadena de la polimerasaes
dc.subjectSensibilidad y especificidades
dc.subject.meshMedical Subject Headings::Named Groups::Persons::Age Groups::Adult::Agedes
dc.subject.meshMedical Subject Headings::Named Groups::Persons::Age Groups::Adult::Aged::Aged, 80 and overes
dc.subject.meshMedical Subject Headings::Organisms::Bacteria::Gram-Negative Bacteria::Gram-Negative Aerobic Bacteria::Gram-Negative Aerobic Rods and Cocci::Brucellaceae::Brucellaes
dc.subject.meshMedical Subject Headings::Diseases::Bacterial Infections and Mycoses::Bacterial Infections::Gram-Negative Bacterial Infections::Brucellosises
dc.subject.meshMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Equipment and Supplies::Culture Mediaes
dc.subject.meshMedical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::DNA::DNA, Bacteriales
dc.subject.meshMedical Subject Headings::Check Tags::Femalees
dc.subject.meshMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humanses
dc.subject.meshMedical Subject Headings::Check Tags::Malees
dc.subject.meshMedical Subject Headings::Named Groups::Persons::Age Groups::Adult::Middle Agedes
dc.subject.meshMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Nucleic Acid Amplification Techniques::Polymerase Chain Reactiones
dc.subject.meshMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Statistics as Topic::Sensitivity and Specificityes
dc.subject.meshMedical Subject Headings::Named Groups::Persons::Age Groups::Adultes
dc.titleDiagnostic yield of a PCR assay in focal complications of
dc.rights.accessRightsAcceso abiertoes
dc.contributor.authoraffiliation[Morata,P; Queipo-Ortuño,I] Department of Biochemistry and Molecular Biology, Faculty of Medicine, Málaga University. [Reguera,JM; Miralles,F; Colmenero,JD] Infectious Diseases Unit, Internal Medicine Department, “Carlos Haya” University Hospital, Málaga, Spain. [Lopez-Gonzalez,JJ] Microbiology Service, “Carlos Haya” University Hospital, Málaga,
Appears in Collections:01- Artículos - Hospital Regional de Málaga

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