Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/2146
Título : Strategy for optimizing DNA amplification in a peripheral blood PCR assay used for diagnosis of human brucellosis.
Autor : Morata, Pilar
Queipo-Ortuño, María Isabel
de Dios Colmenero, Juan de Dios
Filiación: [Morata,P; Queipo-Ortuño,MI] Department of Biochemistry and Molecular Biology, Málaga, University, Málaga, Spain. [Colmenero,J de D] Infectious Diseases Unit, "CarlosHaya" Regional Hospital, Málaga, Spain.
Palabras clave : España
Brucelosis
ADN bacteriano
Electroforesis en gel de agar
Leucocitos
Reacción en cadena de la polimerasa
MeSH: Medical Subject Headings::Organisms::Bacteria::Gram-Negative Bacteria::Gram-Negative Aerobic Bacteria::Gram-Negative Aerobic Rods and Cocci::Brucellaceae::Brucella::Brucella abortus
Medical Subject Headings::Diseases::Bacterial Infections and Mycoses::Bacterial Infections::Gram-Negative Bacterial Infections::Brucellosis
Medical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::DNA::DNA, Bacterial
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Electrophoresis::Electrophoresis, Agar Gel
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Anatomy::Hemic and Immune Systems::Blood::Blood Cells::Leukocytes
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Nucleic Acid Amplification Techniques::Polymerase Chain Reaction
Medical Subject Headings::Geographicals::Geographic Locations::Europe::Spain
Medical Subject Headings::Organisms::Bacteria::Gram-Negative Bacteria::Gram-Negative Aerobic Bacteria::Gram-Negative Aerobic Rods and Cocci::Brucellaceae::Brucella
Fecha de publicación : Sep-1998
Editorial : American Society for Microbiology
Cita Bibliográfica: Morata P, Queipo-Ortuño MI, Colmenero J de D. Strategy for optimizing DNA amplification in a peripheral blood PCR assay used for diagnosis of human brucellosis. J Clin Microbiol. 1998; 36(9):2443-6
Abstract: We studied two of the possible factors which can interfere with specific DNA amplification in a peripheral-blood PCR assay used for the diagnosis of human brucellosis. We found that high concentrations of leukocyte DNA and heme compounds inhibit PCR. These inhibitors can be efficiently suppressed by increasing the number of washings to four or five and decreasing the amount of total DNA to 2 to 4 microg, thereby avoiding false-negative results.
Descripción : Journal Article; Research Support, Non-U.S. Gov't;
URI: http://hdl.handle.net/10668/2146
Versión del editor : http://jcm.asm.org/content/36/9/2443.abstract
ISSN : 0095-1137 (Online)
0095-1137 (Print)
Appears in Collections:01- Artículos - Hospital Regional de Málaga

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