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http://hdl.handle.net/10668/2146| Title: | Strategy for optimizing DNA amplification in a peripheral blood PCR assay used for diagnosis of human brucellosis. |
| Authors: | Morata, Pilar Queipo-Ortuño, María Isabel de Dios Colmenero, Juan de Dios |
| metadata.dc.contributor.authoraffiliation: | [Morata,P; Queipo-Ortuño,MI] Department of Biochemistry and Molecular Biology, Málaga, University, Málaga, Spain. [Colmenero,J de D] Infectious Diseases Unit, "CarlosHaya" Regional Hospital, Málaga, Spain. |
| Keywords: | España;Brucelosis;ADN bacteriano;Electroforesis en gel de agar;Leucocitos;Reacción en cadena de la polimerasa |
| metadata.dc.subject.mesh: | Medical Subject Headings::Organisms::Bacteria::Gram-Negative Bacteria::Gram-Negative Aerobic Bacteria::Gram-Negative Aerobic Rods and Cocci::Brucellaceae::Brucella::Brucella abortus Medical Subject Headings::Diseases::Bacterial Infections and Mycoses::Bacterial Infections::Gram-Negative Bacterial Infections::Brucellosis Medical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::DNA::DNA, Bacterial Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Electrophoresis::Electrophoresis, Agar Gel Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans Medical Subject Headings::Anatomy::Hemic and Immune Systems::Blood::Blood Cells::Leukocytes Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Nucleic Acid Amplification Techniques::Polymerase Chain Reaction Medical Subject Headings::Geographicals::Geographic Locations::Europe::Spain Medical Subject Headings::Organisms::Bacteria::Gram-Negative Bacteria::Gram-Negative Aerobic Bacteria::Gram-Negative Aerobic Rods and Cocci::Brucellaceae::Brucella |
| Issue Date: | Sep-1998 |
| Publisher: | American Society for Microbiology |
| Citation: | Morata P, Queipo-Ortuño MI, Colmenero J de D. Strategy for optimizing DNA amplification in a peripheral blood PCR assay used for diagnosis of human brucellosis. J Clin Microbiol. 1998; 36(9):2443-6 |
| Abstract: | We studied two of the possible factors which can interfere with specific DNA amplification in a peripheral-blood PCR assay used for the diagnosis of human brucellosis. We found that high concentrations of leukocyte DNA and heme compounds inhibit PCR. These inhibitors can be efficiently suppressed by increasing the number of washings to four or five and decreasing the amount of total DNA to 2 to 4 microg, thereby avoiding false-negative results. |
| Description: | Journal Article; Research Support, Non-U.S. Gov't; |
| URI: | http://hdl.handle.net/10668/2146 |
| metadata.dc.relation.publisherversion: | http://jcm.asm.org/content/36/9/2443.abstract |
| ISSN: | 0095-1137 (Online) 0095-1137 (Print) |
| Appears in Collections: | 01- Artículos - Hospital Regional de Málaga |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| MorataP_StrategyForOptimizing.pdf | Artículo publicado | 397,29 kB | Adobe PDF | View/Open |
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