Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/2233
Título : Chronic administration of recombinant IL-6 upregulates lipogenic enzyme expression and aggravates high-fat-diet-induced steatosis in IL-6-deficient mice.
Autor : Vida, Margarita
Gavito, Ana Luisa
Pavón, Francisco Javier
Bautista, Dolores
Serrano, Antonia
Suarez, Juan
Arrabal, Sergio
Decara, Juan
Romero-Cuevas, Miguel
Rodríguez de Fonseca, Fernando
Baixeras, Elena
Filiación: [Vida,M; Gavito,AL; Pavón,FJ; Serrano,A; Suarez,J; Arrabal,S; Decara,J; Romero-Cuevas,M; Rodríguez de Fonseca,F; Baixeras,E] Laboratorio de Investigación, IBIMA/Universidad de Málaga, Málaga, Spain. [Vida,M; Gavito,AL; Pavón,FJ; Serrano,A; Suarez,J; Arrabal,S; Romero-Cuevas,M; Rodríguez de Fonseca,F; Baixeras,E] Centro de Investigación Biomédica en Red de Fisiopatología de la Obesidad y ́ Nutrición (CIBERobn), Instituto de Salud Carlos III (ISCIII) and Ministerio de Ciencia e Innovación (MICINN), Spain. [Vida,M; Gavito,AL; Pavón,FJ; Serrano,A; Suárez,J; Arrabal,S; Decara,J; Rodríguez de Fonseca,F; Baixeras,E] Unidad de Gestión Clínica de Salud Mental, Hospital ́Universitario Regional de Málaga, Málaga, Spain. [Bautista,D] Unidad de Gestión Clínica de Anatomía Patológica, Hospital Universitario Regional de Málaga, Málaga, Spain.
Palabras clave : Liver
Lipogenesis
Steatosis
Interleukin-6
Modelos de enfermedad en animales
Ácido graso sintasa tipo I
Células Hep G2
Interleucina-6
Lipogénesis
Hígado
Ratones
Ratones consanguíneos C57BL
Ratones noqueados
Esteatosis hepática no alcohólica
Fosforilación
Proteínas recombinantes
Factor de transcripción STAT3
Estearoil-CoA desaturasa
Supresor de proteínas señalizadoras de citocinas
Citoquinesis
Carnitina O-palmitoiltransferasa
Acetil-CoA carboxilasa
Alimentación rica en grasa
MeSH: Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Ligases::Carbon-Carbon Ligases::Acetyl-CoA Carboxylase
Medical Subject Headings::Organisms::Eukaryota::Animals
Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Transferases::Acyltransferases::Carnitine Acyltransferases::Carnitine O-Palmitoyltransferase
Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Cycle::Cell Division::Cytokinesis
Medical Subject Headings::Phenomena and Processes::Physiological Phenomena::Nutritional Physiological Phenomena::Diet::Diet, High-Fat
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Models, Animal::Disease Models, Animal
Medical Subject Headings::Anatomy::Cells::Epithelial Cells::Hepatocytes::Hep G2 Cells
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Peptides::Intercellular Signaling Peptides and Proteins::Cytokines::Interleukins::Interleukin-6
Medical Subject Headings::Phenomena and Processes::Metabolic Phenomena::Metabolism::Lipid Metabolism::Lipogenesis
Medical Subject Headings::Anatomy::Digestive System::Liver
Medical Subject Headings::Check Tags::Male
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Mice
Medical Subject Headings::Organisms::Eukaryota::Animals::Animal Population Groups::Animals, Laboratory::Animals, Inbred Strains::Mice, Inbred Strains::Mice, Inbred C57BL
Medical Subject Headings::Organisms::Eukaryota::Animals::Animal Population Groups::Animals, Genetically Modified::Mice, Transgenic::Mice, Knockout
Medical Subject Headings::Phenomena and Processes::Metabolic Phenomena::Metabolism::Phosphorylation
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Recombinant Proteins
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Transcription Factors::STAT Transcription Factors::STAT3 Transcription Factor
Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Oxidoreductases::Oxygenases::Mixed Function Oxygenases::Fatty Acid Desaturases::Stearoyl-CoA Desaturase
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Intracellular Signaling Peptides and Proteins::Adaptor Proteins, Signal Transducing::Suppressor of Cytokine Signaling Proteins
Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Transferases::Phosphotransferases::Phosphotransferases (Alcohol Group Acceptor)::Protein Kinases::Protein-Serine-Threonine Kinases::AMP-Activated Protein Kinases
Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Multifunctional Enzymes::Fatty Acid Synthase, Type I
Medical Subject Headings::Diseases::Digestive System Diseases::Liver Diseases::Fatty Liver::Non-alcoholic Fatty Liver Disease
Fecha de publicación : 1-Jul-2015
Editorial : Company of Biologists
Cita Bibliográfica: Vida M, Gavito AL, Pavón FJ, Bautista D, Serrano A, Suarez J, et al. Chronic administration of recombinant IL-6 upregulates lipogenic enzyme expression and aggravates high-fat-diet-induced steatosis in IL-6-deficient mice. Dis Model Mech. 2015 ; 8(7):721-31
Abstract: Interleukin-6 (IL-6) has emerged as an important mediator of fatty acid metabolism with paradoxical effects in the liver. Administration of IL-6 has been reported to confer protection against steatosis, but plasma and tissue IL-6 concentrations are elevated in chronic liver diseases, including fatty liver diseases associated with obesity and alcoholic ingestion. In this study, we further investigated the role of IL-6 on steatosis induced through a high-fat diet (HFD) in wild-type (WT) and IL-6-deficient (IL-6(-/-)) mice. Additionally, HFD-fed IL-6(-/-) mice were also chronically treated with recombinant IL-6 (rIL-6). Obesity in WT mice fed a HFD associated with elevated serum IL-6 levels, fatty liver, upregulation of carnitine palmitoyltransferase 1 (CPT1) and signal transducer and activator of transcription-3 (STAT3), increased AMP kinase phosphorylation (p-AMPK), and downregulation of the hepatic lipogenic enzymes fatty acid synthase (FAS) and stearoyl-CoA desaturase 1 (SCD1). The HFD-fed IL-6(-/-) mice showed severe steatosis, no changes in CPT1 levels or AMPK activity, no increase in STAT3 amounts, inactivated STAT3, and marked downregulation of the expression of acetyl-CoA carboxylase (ACCα/β), FAS and SCD1. The IL-6 chronic replacement in HFD-fed IL-6 -/-: mice restored hepatic STAT3 and AMPK activation but also increased the expression of the lipogenic enzymes ACCα/β, FAS and SCD1. Furthermore, rIL-6 administration was associated with aggravated steatosis and elevated fat content in the liver. We conclude that, in the context of HFD-induced obesity, the administration of rIL-6 might contribute to the aggravation of fatty liver disease through increasing lipogenesis.
Descripción : Journal Article; Research Support, Non-U.S. Gov't;
URI: http://hdl.handle.net/10668/2233
Versión del editor : http://dmm.biologists.org/content/8/7/721.long
DOI: 10.1242/dmm.019166
ISSN : 1754-8411 (Online)
1754-8403 (Print)
Appears in Collections:01- Artículos - IBIMA. Instituto de Investigación Biomédica de Málaga
01- Artículos - Hospital Regional de Málaga

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