Please use this identifier to cite or link to this item:
Title: Use of MALDI-TOF MS for Identification of Nontuberculous Mycobacterium Species Isolated from Clinical Specimens.
Authors: Mediavilla-Gradolph, María Concepción
De Toro-Peinado, Inmaculada
Bermúdez-Ruiz, María Pilar
García-Martínez, María de los Ángeles
Ortega-Torres, María
Montiel Quezel-Guerraz, Natalia
Palop-Borrás, Begoña
metadata.dc.contributor.authoraffiliation: [Mediavilla-Gradolph,MC; De Toro-Peinado,I; Bermúdez-Ruiz,MP; García-Martínez,MA; Ortega-Torres,M; Palop-Borrás,B] Infectious Diseases and Microbiology Service, Regional University and Virgen de la Victoria University Hospitals, IBIMA, Malaga, Spain. [Montiel Quezel-Guerraz,N] Microbiology Unit, Hospital Costa del Sol, Marbella, Spain.
Keywords: Humanos;Genotipo;Infecciones por micobacterias no tuberculosas;Micobacterias no tuberculosas;Espectrometría de masas con desorción-ionización láser asistida por matriz
metadata.dc.subject.mesh: Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Diseases::Bacterial Infections and Mycoses::Bacterial Infections::Gram-Positive Bacterial Infections::Actinomycetales Infections::Mycobacterium Infections::Mycobacterium Infections, Nontuberculous
Medical Subject Headings::Organisms::Bacteria::Gram-Positive Bacteria::Actinobacteria::Actinomycetales::Mycobacteriaceae::Mycobacterium::Nontuberculous Mycobacteria
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Mass Spectrometry::Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genotype
Issue Date: 2015
Publisher: Hindawi Publishing Corporation
Citation: Mediavilla-Gradolph MC, De Toro-Peinado I, Bermúdez-Ruiz MP, García-Martínez Mde L, Ortega-Torres M, Montiel Quezel-Guerraz N, et al. Use of MALDI-TOF MS for Identification of Nontuberculous Mycobacterium Species Isolated from Clinical Specimens. Biomed Res Int. 2015; 2015:854078
Abstract: The aim of this study was to compare the results obtained for identification by MALDI-TOF of nontuberculous mycobacteria (NTM) isolated in clinical samples with those obtained by GenoType Mycobacterium CM/AS (common mycobacteria/additional species). A total of 66 Mycobacterium isolates from various clinical specimens (mainly respiratory) were tested in this study. They were identified using MALDI-TOF Bruker from strains isolated in Lowenstein, following the recommended protocol of heat inactivation and extraction, and were simultaneously analyzed through hybridization by GenoType Mycobacterium from liquid culture MGIT. Our results showed that identification by MALDI-TOF was correct in 98.4% (65/66) of NTM isolated in our clinical practice (M. avium, M. intracellulare, M. abscessus, M. chelonae, M. fortuitum, M. mucogenicum, M. kansasii, and M. scrofulaceum). MALDI-TOF was found to be an accurate, rapid, and cost-effective system for identification of mycobacteria species.
Description: Journal Article;
metadata.dc.identifier.doi: 10.1155/2015/854078
ISSN: 2314-6141 (Online)
Appears in Collections:01- Artículos - APES Costa del Sol
01- Artículos - Hospital Regional de Málaga
01- Artículos - Hospital Virgen de la Victoria
01- Artículos - IBIMA. Instituto de Investigación Biomédica de Málaga

Files in This Item:
File Description SizeFormat 
Mediavilla_UseOfMaldiTof.pdfArtículo publicado1,47 MBAdobe PDFView/Open

This item is protected by original copyright

This item is licensed under a Creative Commons License Creative Commons