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http://hdl.handle.net/10668/2240| Title: | Use of MALDI-TOF MS for Identification of Nontuberculous Mycobacterium Species Isolated from Clinical Specimens. |
| Authors: | Mediavilla-Gradolph, María Concepción De Toro-Peinado, Inmaculada Bermúdez-Ruiz, María Pilar García-Martínez, María de los Ángeles Ortega-Torres, María Montiel Quezel-Guerraz, Natalia Palop-Borrás, Begoña |
| metadata.dc.contributor.authoraffiliation: | [Mediavilla-Gradolph,MC; De Toro-Peinado,I; Bermúdez-Ruiz,MP; García-Martínez,MA; Ortega-Torres,M; Palop-Borrás,B] Infectious Diseases and Microbiology Service, Regional University and Virgen de la Victoria University Hospitals, IBIMA, Malaga, Spain. [Montiel Quezel-Guerraz,N] Microbiology Unit, Hospital Costa del Sol, Marbella, Spain. |
| Keywords: | Humanos;Genotipo;Infecciones por micobacterias no tuberculosas;Micobacterias no tuberculosas;Espectrometría de masas con desorción-ionización láser asistida por matriz |
| metadata.dc.subject.mesh: | Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans Medical Subject Headings::Diseases::Bacterial Infections and Mycoses::Bacterial Infections::Gram-Positive Bacterial Infections::Actinomycetales Infections::Mycobacterium Infections::Mycobacterium Infections, Nontuberculous Medical Subject Headings::Organisms::Bacteria::Gram-Positive Bacteria::Actinobacteria::Actinomycetales::Mycobacteriaceae::Mycobacterium::Nontuberculous Mycobacteria Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Mass Spectrometry::Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genotype |
| Issue Date: | 2015 |
| Publisher: | Hindawi Publishing Corporation |
| Citation: | Mediavilla-Gradolph MC, De Toro-Peinado I, Bermúdez-Ruiz MP, García-Martínez Mde L, Ortega-Torres M, Montiel Quezel-Guerraz N, et al. Use of MALDI-TOF MS for Identification of Nontuberculous Mycobacterium Species Isolated from Clinical Specimens. Biomed Res Int. 2015; 2015:854078 |
| Abstract: | The aim of this study was to compare the results obtained for identification by MALDI-TOF of nontuberculous mycobacteria (NTM) isolated in clinical samples with those obtained by GenoType Mycobacterium CM/AS (common mycobacteria/additional species). A total of 66 Mycobacterium isolates from various clinical specimens (mainly respiratory) were tested in this study. They were identified using MALDI-TOF Bruker from strains isolated in Lowenstein, following the recommended protocol of heat inactivation and extraction, and were simultaneously analyzed through hybridization by GenoType Mycobacterium from liquid culture MGIT. Our results showed that identification by MALDI-TOF was correct in 98.4% (65/66) of NTM isolated in our clinical practice (M. avium, M. intracellulare, M. abscessus, M. chelonae, M. fortuitum, M. mucogenicum, M. kansasii, and M. scrofulaceum). MALDI-TOF was found to be an accurate, rapid, and cost-effective system for identification of mycobacteria species. |
| Description: | Journal Article; |
| URI: | http://hdl.handle.net/10668/2240 |
| metadata.dc.relation.publisherversion: | http://www.hindawi.com/journals/bmri/2015/854078/abs/ |
| metadata.dc.identifier.doi: | 10.1155/2015/854078 |
| ISSN: | 2314-6141 (Online) |
| Appears in Collections: | 01- Artículos - APES Costa del Sol 01- Artículos - Hospital Regional de Málaga 01- Artículos - Hospital Virgen de la Victoria 01- Artículos - IBIMA. Instituto de Investigación Biomédica de Málaga |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Mediavilla_UseOfMaldiTof.pdf | Artículo publicado | 1,47 MB | Adobe PDF | View/Open |
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