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http://hdl.handle.net/10668/2247| Title: | Derivation of HVR1, HVR2 and HVR3 human embryonic stem cell lines from IVF embryos after preimplantation genetic diagnosis (PGD) for monogenic disorder. |
| Authors: | Hmadcha, Abdelkrim Aguilera, Yolanda Lozano-Arana, Maria Dolores Mellado, Nuria Sánchez, Javier Moya, Cristina Sánchez-Palazón, Luis Palacios, Jose Antiñolo, Guillermo Soria, Bernat |
| metadata.dc.contributor.authoraffiliation: | [Hmadcha,A; Aguilera,Y; Mellado,N; Sánchez-Palazón,L; Soria,B] Department of Stem Cells, Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER), Seville, Spain. [Hmadcha,A; Soria,B] Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólica asociada (CIBERDEM), Madrid, Spain. [Lozano-Arana,MD; Sánchez,J; Moya,C; Antiñolo,G] Department of Genetics, Reproduction and Fetal Medicine, and [Palacios,J] Department of Pathology, Instituto de Biomedicina de Sevilla (IBiS), Seville, Spain. [Palacios,J] Department of Pathology, Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain. [Antiñolo,G] Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), Madrid, Spain |
| Keywords: | Células madre embrionarias;Fertilización in Vitro;Investigación básica;Investigaciones con embriones |
| metadata.dc.subject.mesh: | Medical Subject Headings::Anatomy::Cells::Stem Cells::Embryonic Stem Cells::Human Embryonic Stem Cells Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Reproductive Techniques::Reproductive Techniques, Assisted::Fertilization in Vitro Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Diagnostic Techniques, Obstetrical and Gynecological::Preimplantation Diagnosis Medical Subject Headings::Anatomy::Cells::Cells, Cultured::Cell Line Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Embryo Research |
| Issue Date: | May-2016 |
| Publisher: | Elsevier |
| Citation: | Hmadcha A, Aguilera Y, Lozano-Arana MD, Mellado N, Sánchez J, Moya C, et al. Derivation of HVR1, HVR2 and HVR3 human embryonic stem cell lines from IVF embryos after preimplantation genetic diagnosis (PGD) for monogenic disorder. Stem Cell Res. 2016;16(3):635-9 |
| Abstract: | From 106 human blastocyts donate for research after in vitro fertilization (IVF) and preimplantation genetic diagnosis (PGD) for monogenetic disorder, 3 human embryonic stem cells (hESCs) HVR1, HVR2 and HVR3 were successfully derived. HVR1 was assumed to be genetically normal, HVR2 carrying Becker muscular dystrophy and HVR3 Hemophilia B. Despite the translocation t(9;15)(q34.3;q14) detected in HVR2, all the 3 cell lines were characterised in vitro and in vivo as normal hESCs lines and were registered in the Spanish Stem Cell Bank. |
| Description: | Lab resource |
| URI: | http://hdl.handle.net/10668/2247 |
| metadata.dc.relation.publisherversion: | http://www.sciencedirect.com/science/article/pii/S1873506116300101 |
| metadata.dc.identifier.doi: | 10.1016/j.scr.2016.03.012 |
| ISSN: | 1873-5061 |
| Appears in Collections: | Centro Andaluz de Biología Molecular (CABIMER) Instituto de Investigación Biomédica de Sevilla (IBIS) |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Hmadcha_DerivationOfHVR1.pdf | Artículo publicado | 1,4 MB | Adobe PDF | View/Open |
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