Please use this identifier to cite or link to this item: http://hdl.handle.net/10668/2248
Título : Natural Killer Cells Improve Hematopoietic Stem Cell Engraftment by Increasing Stem Cell Clonogenicity In Vitro and in a Humanized Mouse Model.
Autor : Escobedo-Cousin, Michelle
Jackson, Nicola
Laza-Briviesca, Raquel
Ariza-McNaughton, Linda
Luevano, Martha
Derniame, Sophie
Querol, Sergio
Blundell, Michael
Thrasher, Adrian
Soria, Bernat
Cooper, Nichola
Bonnet, Dominique
Madrigal, Alejandro
Saudemont, Aurore
Filiación: [Escobedo-Cousin,M; Luevano,M; Derniame,S; Madrigal,A; Saudemont,A] University College London, Cancer Institute, London, United Kingdom. [Escobedo-Cousin,M; Jackson,N; Laza-Briviesca,R; Luevano,M; Derniame,S; Madrigal,A; Saudemont,A] Anthony Nolan Research Institute, Royal Free Campus, London, United Kingdom. [Ariza- McNaughton,L; Bonnet,D] Cancer Research UK, London Research Institute, London, United Kingdom. [Querol,S] Programa Concordia Banc de Sang i Teixits, Barcelona, Spain. [Blundell,M; Thrasher,A] University College London, Institute of Child Health, London, United Kingdom. [Soria,B] Andalusian Center for Molecular Biology and Regenerative Medicine (CABIMER), Fundación Progreso y Salud, Seville, Spain; and Biomedical Research Network on Diabetes and Related Metabolic Diseases (CIBERDEM), Instituto de Salud Carlos III, Madrid, Spain. [Cooper,D] Haematology Department, Hammersmith Hospital, London, United Kingdom
Palabras clave : NK cells
Hematopoietic stem cells
Mouse models
Integrins
T cells
Gene expression
Flow cytometry
Cytotoxic T cells
Células Asesinas Naturales
Células Madre Hematopoyéticas
Células Madre Embrionarias de Ratones
Integrinas
Expresión Génica
Citometría de flujo
MeSH: Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Movement
Medical Subject Headings::Chemicals and Drugs::Biological Factors::Chemotactic Factors::Chemokines::Chemokines, CXC::Chemokine CXCL9
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Surgical Procedures, Operative::Transplantation::Cell Transplantation::Stem Cell Transplantation::Cord Blood Stem Cell Transplantation
Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Cycle::Cell Division::Cytokinesis
Medical Subject Headings::Anatomy::Embryonic Structures::Fetus::Fetal Blood
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Gene Expression Regulation
Medical Subject Headings::Diseases::Immune System Diseases::Graft vs Host Disease
Medical Subject Headings::Phenomena and Processes::Immune System Phenomena::Immune System Processes::Transplantation Immunology::Graft vs Host Reaction::Graft vs Tumor Effect::Graft vs Leukemia Effect
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Surgical Procedures, Operative::Transplantation::Cell Transplantation::Stem Cell Transplantation::Hematopoietic Stem Cell Transplantation
Medical Subject Headings::Anatomy::Hemic and Immune Systems::Hematopoietic System::Bone Marrow Cells::Hematopoietic Stem Cells
Medical Subject Headings::Chemicals and Drugs::Biological Factors::Intercellular Signaling Peptides and Proteins::Cytokines::Interleukins::Interleukin-15
Medical Subject Headings::Anatomy::Cells::Blood Cells::Leukocytes::Leukocytes, Mononuclear::Lymphocytes::Killer Cells, Natural
Medical Subject Headings::Anatomy::Cells::Blood Cells::Leukocytes::Leukocytes, Mononuclear
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Mice
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Mice::Mice, Inbred Strains::Mice, Inbred NOD
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Mice::Mice, Mutant Strains::Mice, SCID
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Nucleic Acid Hybridization::Oligonucleotide Array Sequence Analysis
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Recombinant Proteins
Medical Subject Headings::Anatomy::Cells::Stem Cells
Animals
Fecha de publicación : 2015
Editorial : Public Library of Science
Cita Bibliográfica: Escobedo-Cousin M, Jackson N, Laza-Briviesca R, Ariza-McNaughton L, Luevano M, Derniame S, et al. Natural Killer Cells Improve Hematopoietic Stem Cell Engraftment by Increasing Stem Cell Clonogenicity In Vitro and in a Humanized Mouse Model. PLoS ONE; 10(10):e0138623
Abstract: Cord blood (CB) is increasingly used as a source of hematopoietic stem cells (HSC) for transplantation. Low incidence and severity of graft-versus-host disease (GvHD) and a robust graft-versus-leukemia (GvL) effect are observed following CB transplantation (CBT). However, its main disadvantages are a limited number of HSC per unit, delayed immune reconstitution and a higher incidence of infection. Unmanipulated grafts contain accessory cells that may facilitate HSC engraftment. Therefore, the effects of accessory cells, particularly natural killer (NK) cells, on human CB HSC (CBSC) functions were assessed in vitro and in vivo. CBSC cultured with autologous CB NK cells showed higher levels of CXCR4 expression, a higher migration index and a higher number of colony forming units (CFU) after short-term and long-term cultures. We found that CBSC secreted CXCL9 following interaction with CB NK cells. In addition, recombinant CXCL9 increased CBSC clonogenicity, recapitulating the effect observed of CB NK cells on CBSC. Moreover, the co-infusion of CBSC with CB NK cells led to a higher level of CBSC engraftment in NSG mouse model. The results presented in this work offer the basis for an alternative approach to enhance HSC engraftment that could improve the outcome of CBT.
Descripción : Journal Article; Research Support, Non-U.S. Gov't;
URI: http://hdl.handle.net/10668/2248
Versión del editor : http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0138623
DOI: 10.1371/journal.pone.0138623
ISSN : 1932-6203 (Online)
Appears in Collections:01- Artículos - CABIMER. Centro Andaluz de Biología Molecular

Files in This Item:
File Description SizeFormat 
Escobedo_NaturalKillerCells.pdfArtículo publicado1,93 MBAdobe PDFView/Open
Escobedo_NaturalKillerCells_Fig1.PNGFigura 1298,99 kBimage/pngThumbnail
View/Open
Escobedo_NaturalKillerCells_Fig2.PNGFigura 265,92 kBimage/pngThumbnail
View/Open
Escobedo_NaturalKillerCells_Fig3.PNGFigura 366,65 kBimage/pngThumbnail
View/Open
Escobedo_NaturalKillerCells_Fig4.PNGFigura 4554,68 kBimage/pngThumbnail
View/Open
Escobedo_NaturalKillerCells_Fig5.PNGFigura 5226,39 kBimage/pngThumbnail
View/Open
Escobedo_NaturalKillerCells_Fig6.PNGFigura 6771,96 kBimage/pngThumbnail
View/Open
Escobedo_NaturalKillerCells_Fig7.PNGFigura 7428,31 kBimage/pngThumbnail
View/Open
Escobedo_NaturalKillerCells_T1.DOCXTabla 1355,33 kBMicrosoft Word XMLView/Open
Escobedo_NaturalKillerCells_T2.DOCXTabla 272,14 kBMicrosoft Word XMLView/Open


This item is licensed under a Creative Commons License Creative Commons